Insulin-degrading enzyme, Ide ELISA KIT/ Mouse Insulin-degrading enzyme, Ide ELISA试剂盒

Product Name:Mouse Insulin- degrading enzyme, Ide ELISA KIT
Packing:96T

Catalog No.:ELI-06094m

Gene Name:Mouse Ide

Detect Range:78.1-5000pg/ml

Sensitivity:46.875pg/ml

Target Protein Name:Mouse Ide

Alternative Name:Mouse Insulin-degrading enzyme, Ide
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Insulin- degrading enzyme, Ide ELISA KIT allows for the in vitro quantitative determination of Mouse Ide concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Insulin- degrading enzyme, Ide ELISA KIT has been pre-coated with an Mouse Insulin-degrading enzyme, Ide antibody specific to Mouse Ide .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ide and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ide , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ide in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

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