Nuclear factor NF-kappa-B p105 subunit, Nfkb1 ELISA KIT/ Mouse Nuclear factor NF-kappa-B p105 subunit, Nfkb1 ELISA试剂盒

Mouse Nuclear factor NF- kappa- B p105 subunit, NFKB1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

p50,nuclear factor NF-kappa-B p50 subunit; NFKB-p50; EBP-1; KBF1; NF-kB1; NF-kappa-B; NF-kappaB; NFKB-p105; NFkappaB; p105; DNA binding factor KBF1; DNA-binding factor KBF1; NF-kappabeta; nuclear factor NF-kappa-B p105 subunit; nuclear factor kappa-B DNA binding subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

Search name

Mouse p50 ELISA KIT ,Mouse nuclear factor NF-kappa-B p50 subunit ELISA KIT ,Mouse NFKB-p50 ELISA KIT ,Mouse EBP-1 ELISA KIT ,Mouse KBF1 ELISA KIT ,Mouse NF-kB1 ELISA KIT ,Mouse NF-kappa-B ELISA KIT ,Mouse NF-kappaB ELISA KIT ,Mouse NFKB-p105 ELISA KIT ,Mouse NFkappaB ELISA KIT ,Mouse p105 ELISA KIT ,Mouse DNA binding factor KBF1 ELISA KIT ,Mouse DNA-binding factor KBF1 ELISA KIT ,Mouse NF-kappabeta ELISA KIT ,Mouse nuclear factor NF-kappa-B p105 subunit ELISA KIT ,Mouse nuclear factor kappa-B DNA binding subunit ELISA KIT ,Mouse nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse Nuclear factor NF-kappa-B p105 subunit concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear factor NF-kappa-B p105 subunit. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Nuclear factor NF-kappa-B p105 subunit and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Nuclear factor NF-kappa-B p105 subunit, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Nuclear factor NF-kappa-B p105 subunit in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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