Serum amyloid A-3 protein, Saa3 ELISA KIT/ Mouse Serum amyloid A-3 protein, Saa3 ELISA试剂盒

Mouse Serum amyloid A- 3 protein, Saa3 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY!

Alternative name

Mouse Saa3 ELISA KIT ,Mouse Serum amyloid A-3 protein ELISA KIT ,Mouse AV098916 ELISA KIT ,Mouse Saa-3 ELISA KIT ,Mouse l7R3 ELISA KIT ,Mouse serum amyloid A 3 ELISA KIT

Intended use

Mouse Saa3 ELISA KIT t allows for the in vitro quantitative determination of mouse serum amyloid a-3 protein concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Mouse Saa3 ELISA KIT has been pre-coated with an antibody specific to Serum amyloid A-3 protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Serum amyloid A-3 protein and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Serum amyloid A-3 protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Serum amyloid A-3 protein in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Mouse Saa3 ELISA KIT Range 0.15-10ng/ml

Mouse Saa3 ELISA KIT Manual download

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