Adenosine deaminase, Ada ELISA KIT/ Mouse Adenosine deaminase, Ada ELISA试剂盒

Mouse Adenosine deaminase, ADA ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Adenosine deaminase,Adenosine aminohydrolase,ADA

Search name
Mouse Adenosine deaminase ELISA Kit, Mouse ADA ELISA Kit,Adenosine deaminase ELISA Kit,ADA ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse adenosine deaminase,Adenosine deaminase
concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenosine deaminase. Standards or samples are
then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Adenosine
deaminase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB
substrate solution is added to each well. Only those wells that contain Adenosine deaminase, biotin-conjugated antibody and
enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid
solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Adenosine
deaminase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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