Delta-like protein 4, Dll4 ELISA KIT/ Mouse Delta-like protein 4, Dll4 ELISA试剂盒

Mouse Delta- like protein 4, DLL4 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

DLL4,notch ligand DLL4; hdelta2; delta 4; delta ligand 4; delta-like 4 homolog; delta-like 4 protein; delta-like protein 4; delta4; drosophila Delta homolog 4; notch ligand delta-2; delta-like 4 (Drosophila)

Search name

Mouse DLL4 ELISA KIT ,Mouse notch ligand DLL4 ELISA KIT ,Mouse hdelta2 ELISA KIT ,Mouse delta 4 ELISA KIT ,Mouse delta ligand 4 ELISA KIT ,Mouse delta-like 4 homolog ELISA KIT ,Mouse delta-like 4 protein ELISA KIT ,Mouse delta-like protein 4 ELISA KIT ,Mouse delta4 ELISA KIT ,Mouse drosophila Delta homolog 4 ELISA KIT ,Mouse notch ligand delta-2 ELISA KIT ,Mouse delta-like 4 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse Delta- like protein 4, DLL4 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Delta- like protein 4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Delta- like protein 4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Delta- like protein 4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Delta- like protein 4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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