Glucagon, Gcg ELISA KIT/ Mouse Glucagon, Gcg ELISA试剂盒

Mouse Glucagon, GCG ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Glucagon,glucagon-like peptide 2; glucagon-like peptide 1; GLP1; GLP2; GRPP; glicentin-related polypeptide,GcG, preproglucagon

Search name
Mouse Glucagon ELISA Kit, Mouse glucagon-like peptide 2 ELISA Kit, Mouse glucagon-like peptide 1 ELISA Kit, Mouse GLP1
ELISA Kit, Mouse GLP2 ELISA Kit, Mouse GRPP ELISA Kit, Mouse GC ELISA Kit, Mouse glicentin-related polypeptide ELISA
Kit, Mouse preproglucagon ELISA KIT,Mouse GCG ELISA KIT

Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse glucagon,Glucagon concentrations in serum, Plasma,
tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucagon. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Glucagon and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain Glucagon, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color.
The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Glucagon in the samples is then determined by
comparing the O.D. of the samples to the standard curve.

PDF manual download

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