Inter-alpha-trypsin inhibitor heavy chain H1, Itih1 ELIS/ Mouse Inter-alpha-trypsin inhibitor heavy chain H1, Itih1 ELIS

Mouse Inter- alpha- trypsin inhibitor heavy chain H1, ITIH1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

ITIH1,H1P; IATIH; IGHEP1; ITI-HC1; ITIH; SHAP; ITI heavy chain H1; inter-alpha (globulin) inhibitor H1; inter-alpha (globulin) inhibitor, H1 polypeptide; inter-alpha-inhibitor heavy chain 1; inter-alpha-trypsin inhibitor complex component III; inter-alpha-trypsin inhibitor heavy chain H1; serum-derived hyaluronan-associated protein; inter-alpha-trypsin inhibitor heavy chain 1

Search name

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Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse Inter- alpha- trypsin inhibitor heavy chain H1, ITIH1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inter-alpha-trypsin inhibitor heavy chain H1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Inter-alpha-trypsin inhibitor heavy chain H1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Inter-alpha-trypsin inhibitor heavy chain H1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Inter-alpha-trypsin inhibitor heavy chain H1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Customized product

Delivery time 4-6month

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