Mouse Calbindin, CALB ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Calbindin,Vitamin D-dependent calcium-binding protein, avian-type,Calbindin D28,D-28K, calbindin,D-28K,CAB27,CALB; D-28K; RTVL-H protein; calbindin 1, (28kD); calbindin D28; vitamin D-dependent calcium-binding protei, avian-type; calbindin 1 28kDa
Search name
Mouse Calbindin ELISA KIT ,Mouse Vitamin D-dependent calcium-binding protein ELISA KIT ,Mouse avian-type ELISA KIT ,Mouse Calbindin D28 ELISA KIT ,Mouse D-28K ELISA KIT ,Mouse calbindin ELISA KIT ,Mouse D-28K ELISA KIT ,Mouse CAB27 ELISA KIT ,Mouse CALB ELISA KIT ,Mouse D-28K ELISA KIT ,Mouse RTVL-H protein ELISA KIT ,Mouse calbindin 1 (28kD) ELISA KIT ,Mouse calbindin D28 ELISA KIT ,Mouse vitamin D-dependent calcium-binding protei ELISA KIT ,Mouse avian-type ELISA KIT ,Mouse calbindin 1 28kDa ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse calbindin,Calbindin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calbindin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Calbindin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Calbindin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Calbindin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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