Tryptase, Tpsab1 ELISA KIT/ Mouse Tryptase, Tpsab1 ELISA试剂盒

Mouse Tryptase, Tpsab1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

TPS,Tpsab1,Tryptase,tryptase alpha/beta 1; tryptase skin; tryptase beta 1; tryptase alpha/beta-1; Mcpt7; TPSB1; rMCP-7; MMCP-7; mast cell protease 7

Search name

Mouse TPS ELISA KIT,Mouse Tpsab1 ELISA KIT ,Mouse Tryptase ELISA KIT ,Mouse tryptase alpha/beta 1 ELISA KIT ,Mouse tryptase skin ELISA KIT ,Mouse tryptase beta 1 ELISA KIT ,Mouse tryptase alpha/beta-1 ELISA KIT ,Mouse Mcpt7 ELISA KIT ,Mouse TPSB1 ELISA KIT ,Mouse rMCP-7 ELISA KIT ,Mouse MMCP-7 ELISA KIT ,Mouse mast cell protease 7 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse tryptase,Tryptase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tryptase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Tryptase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Tryptase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Tryptase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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