Vitronectin, Vtn ELISA KIT/ Mouse Vitronectin, Vtn ELISA试剂盒

Mouse Vitronectin, VNT ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Vitronectin,S-protein,Serum-spreading factor, V75; VNT; S-protein; complement S-protein; epibolin; serum spreading factor;
serum-spreading factor; somatomedin B

Search name
Mouse Vitronectin ELISA Kit, Mouse VTN ELISA Kit, Mouse S-protein ELISA Kit, Vitronectin ELISA Kit, VTN ELISA Kit, S-protein
ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse vitronectin,Vitronectin concentrations in serum, Plasma,
Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitronectin. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Vitronectin and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain Vitronectin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in
color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Vitronectin in the samples is then determined by
comparing the O.D. of the samples to the standard curve.

PDF manual download

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