Platelet-activating factor acetylhydrolase, Pla2g7 ELISA / Mouse Platelet-activating factor acetylhydrolase, Pla2g7 ELISA

Mouse Platelet- activating factor acetylhydrolase, PLA2G7 ELISA KIT

96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Platelet-activating factor acetylhydrolase(PAF acetylhydrolase),PAF 2-acylhydrolase,LDL-associated phospholipase
A2(LDL-PLA(2)),Group-VIIA phospholipase A2(gVIIA-PLA2),1-alkyl-2-acetylglycerophosphocholine
esterase,2-acetyl-1-alkylglycerophosphocholine esterase,
lipoprotein-associated phospholipase A2,LDL-PLA2,LP-PLA2,PAFAD,PAFAH,1-alkyl-2-acetylglycerophosphocholine
esterase,2-acetyl-1-alkylglycerophosphocholine esterase,LDL-PLA(2),LDL-associated phospholipase A2,PAF 2-acylhydrolase,PAF
acetylhydrolase,gVIIA-PLA2,group-VIIA phospholipase A2,platelet-activating factor acetylhydrolase,phospholipase A2, group VII
(platelet-activating factor acetylhydrolase, plasma)

Search name
Mouse lipoprotein-associated phospholipase A2 ELISA Kit,Mouse LP-PLA2 ELISA Kit, lipoprotein-associated phospholipase
A2 ELISA Kit,LP-PLA2 ELISA Kit, Mouse Platelet-activating factor acetylhydrolase ELISA Kit, Mouse PAF acetylhydrolase
ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse platelet-activating factor acetylhydrolase,PAF
acetylhydrolase concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to PAF acetylhydrolase, During the reaction, PAF acetylhydrolase in the sample or standard
competes with a fixed amount of biotin-labeled PAF acetylhydrolase for sites on a pre-coated Monoclonal antibody specific to PAF
acetylhydrolase. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish
Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PAF acetylhydrolase in the samples is then determined by
comparing the O.D. of the samples to the standard curve.

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