Glycogen phosphorylase, brain form, Pygb ELISA KIT/ Mouse Glycogen phosphorylase, brain form, Pygb ELISA试剂盒

Mouse Glycogen phosphorylase, brain form, PYGB ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

PYGB,GPBB; glycogen phosphorylase B; phosphorylase glycogen brain, Glycogen phosphorylase brain form

Search name

Mouse PYGB ELISA KIT ,Mouse GPBB ELISA KIT ,Mouse glycogen phosphorylase B ELISA KIT ,Mouse phosphorylase glycogen brain ELISA KIT ,Mouse Glycogen phosphorylase brain form ELISA KIT ,Mouse Glycogen phosphorylase brain ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse Glycogen phosphorylase, brain form concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycogen phosphorylase, brain form. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Glycogen phosphorylase, brain form and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Glycogen phosphorylase, brain form, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Glycogen phosphorylase, brain form in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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