von Willebrand factor, Vwf ELISA KIT/ Mouse von Willebrand factor, Vwf ELISA试剂盒

Mouse von Willebrand factor, vWF ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
von Willebrand factor,vWF, F8VWF,VWD,coagulation factor VIII VWF

Search name
Mouse von Willebrand factor ELISA Kit,Mouse vWF ELISA Kit,von Willebrand factor ELISA Kit,vWF ELISA Kit,ELISA Kit,von
Willebrand factor,vWF, F8VWF,VWD,coagulation factor VIII VWF

Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse von willebrand factor,vWF concentrations in serum,
Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to vWF. Standards or samples are then added to the
appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for vWF and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain vWF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of vWF in the samples is then determined by comparing the
O.D. of the samples to the standard curve.

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