Mouse Proteinase- activated receptor 1, F2r ELISA KIT Product Name:Mouse Proteinase- activated receptor 1, F2r ELISA KIT
Packing:96T
Catalog No.:ELI-02796m
Gene Name:Mouse F2r
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse F2r
Alternative Name:Mouse Proteinase-activated receptor 1, F2r
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Proteinase- activated receptor 1, F2r ELISA KIT allows for the in vitro quantitative determination of Mouse F2r concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Proteinase- activated receptor 1, F2r ELISA KIT has been pre-coated with an Mouse Proteinase-activated receptor 1, F2r antibody specific to Mouse F2r .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse F2r and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse F2r , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse F2r in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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