Mouse T- lymphocyte activation antigen CD86, Cd86 ELISA KIT Product Name:Mouse T- lymphocyte activation antigen CD86, Cd86 ELISA KIT
Packing:96T
Catalog No.:ELI-02791m
Gene Name:Mouse Cd86
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Cd86
Alternative Name:Mouse T-lymphocyte activation antigen CD86, Cd86
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse T- lymphocyte activation antigen CD86, Cd86 ELISA KIT allows for the in vitro quantitative determination of Mouse Cd86 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse T- lymphocyte activation antigen CD86, Cd86 ELISA KIT has been pre-coated with an Mouse T-lymphocyte activation antigen CD86, Cd86 antibody specific to Mouse Cd86 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cd86 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cd86 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cd86 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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