Hypoxia-inducible factor 1-alpha, Hif1a ELISA KIT/ Mouse Hypoxia-inducible factor 1-alpha, Hif1a ELISA试剂盒

Mouse Hypoxia- inducible factor 1- alpha, HIF1A ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

HIF1A,HIF-1 alpha,HIF-1-alpha,HIF-1A,HIF-1alpha,HIF1,HIF1-ALPHA,MOP1,PASD8,bHLHe78,Hypoxia-inducible factor 1-alpha,HIF1-alpha,ARNT interacting protein,ARNT-interacting protein,PAS domain-containing protein 8,basic-helix-loop-helix-PAS protein MOP1,class E basic helix-loop-helix protein 78,hypoxia inducible factor 1 alpha subunit,basic helix-loop-helix transcription factor,hypoxia-inducible factor 1 alpha isoform I.3,hypoxia-inducible factor 1 alpha subunit,basic helix-loop-helix transcription factor,hypoxia-inducible factor1alpha,member of PAS protein 1,member of PAS superfamily 1,hypoxia inducible factor 1 alpha subunit

Search name

Mouse HIF1A ELISA KIT ,Mouse HIF-1 alpha ELISA KIT ,Mouse HIF-1-alpha ELISA KIT ,Mouse HIF-1A ELISA KIT ,Mouse HIF-1alpha ELISA KIT ,Mouse HIF1 ELISA KIT ,Mouse HIF1-ALPHA ELISA KIT ,Mouse MOP1 ELISA KIT ,Mouse PASD8 ELISA KIT ,Mouse bHLHe78 ELISA KIT ,Mouse Hypoxia-inducible factor 1-alpha ELISA KIT ,Mouse HIF1-alpha ELISA KIT ,Mouse ARNT interacting protein ELISA KIT ,Mouse ARNT-interacting protein ELISA KIT ,Mouse PAS domain-containing protein 8 ELISA KIT ,Mouse basic-helix-loop-helix-PAS protein MOP1 ELISA KIT ,Mouse class E basic helix-loop-helix protein 78 ELISA KIT ,Mouse hypoxia inducible factor 1 alpha subunit ELISA KIT ,Mouse basic helix-loop-helix transcription factor ELISA KIT ,Mouse hypoxia-inducible factor 1 alpha isoform I.3 ELISA KIT ,Mouse hypoxia-inducible factor 1 alpha subunit ELISA KIT ,Mouse basic helix-loop-helix transcription factor ELISA KIT ,Mouse hypoxia-inducible factor1alpha ELISA KIT ,Mouse member of PAS protein 1 ELISA KIT ,Mouse member of PAS superfamily 1 ELISA KIT ,Mouse hypoxia inducible factor 1 alpha subunit ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse hypoxia-inducible factor 1-alpha,HIF-1-alpha concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to HIF-1-alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HIF-1-alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HIF-1-alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HIF-1-alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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