Angiopoietin-4, Angpt4 ELISA KIT/ Mouse Angiopoietin-4, Angpt4 ELISA试剂盒

Mouse Angiopoietin- 4, ANGPT4 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

ANGPT4,AGP4; ANG-3; ANG4; ANG-4; angiopoietin-3; angiopoietin-4; dJ824F16.2; angiopoietin 4

Search name

Mouse ANGPT4 ELISA KIT ,Mouse AGP4 ELISA KIT ,Mouse ANG-3 ELISA KIT ,Mouse ANG4 ELISA KIT ,Mouse ANG-4 ELISA KIT ,Mouse angiopoietin-3 ELISA KIT ,Mouse angiopoietin-4 ELISA KIT ,Mouse dJ824F16.2 ELISA KIT ,Mouse angiopoietin 4 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse angiopoietin-4,ANG-4 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to ANG-4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ANG-4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ANG-4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ANG-4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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