Mouse Octamer Binding Transcription Factor 7 ELISA Kit Product Name:Mouse Octamer Binding Transcription Factor 7 ELISA Kit
Packing:96T
Catalog No.:ELA-E1614m
Gene Name:Mouse OCT7
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse OCT7
Alternative Name:Mouse Octamer Binding Transcription Factor 7
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Octamer Binding Transcription Factor 7 ELISA Kit allows for the in vitro quantitative determination of Mouse OCT7 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Octamer Binding Transcription Factor 7 ELISA Kit has been pre-coated with an Mouse Octamer Binding Transcription Factor 7 antibody specific to Mouse OCT7 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse OCT7 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse OCT7 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse OCT7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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