corticotropin releasing hormone ELISA Kit/ Mouse corticotropin releasing hormone ELISA试剂盒

Mouse corticotropin releasing hormone,CRH ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Corticoliberin,Corticotropin-releasing factor(CRF),Corticotropin-releasing hormone,

Search name
Mouse corticotropin releasing hormone ELISA Kit, Mouse CRH ELISA Kit, Mouse CRF ELISA Kit, Mouse Corticotropin-releasing
factor ELISA Kit, Mouse Corticoliberin ELISA Kit, corticotropin releasing hormone ELISA Kit, CRH ELISA Kit, CRF ELISA Kit,
Corticotropin-releasing factor ELISA Kit, Corticoliberin ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse Corticoliberin concentrations in serum, Plasma, Urine,
tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Corticoliberin. Standards or samples are then
added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Corticoliberin and
Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is
added to each well. Only those wells that contain Corticoliberin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a
change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is
measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Corticoliberin in the samples is then
determined by comparing the O.D. of the samples to the standard curve.

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