Phosphatidylinositol 4,5-bisphosphate, PIP2 ELISA Kit/ Mouse Phosphatidylinositol 4,5-bisphosphate, PIP2 ELISA试剂盒

Mouse PtdIns (4, 5) P2, PIP2 ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY!

Synonyms
Phosphatidylinositol 4,5-bisphosphate, PtdIns(4,5)P2, PIP2

Search name
Mouse PIP2 ELISA Kit,Mouse Phosphatidylinositol 4,5-bisphosphate ELISA Kit, Mouse PtdIns(4,5)P2 ELISA Kit,

Intended use
Mouse PIP2 ELISA Kit allows for the in vitro quantitative determination of Mouse Phosphatidylinositol 4,5-bisphosphate concentrations in
serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
Mouse PIP2 ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Phosphatidylinositol 4,5-bisphosphate, During the reaction, Phosphatidylinositol 4,5-bisphosphate
in the sample or standard competes with a fixed amount of biotin-labeled Phosphatidylinositol 4,5-bisphosphate for sites on a pre-coated
Monoclonal antibody specific to Phosphatidylinositol 4,5-bisphosphate. Excess conjugate and unbound sample or standard are washed
from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB
substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the
color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Phosphatidylinositol
4,5-bisphosphate in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 0.78-50ng/ml

Mouse PIP2 ELISA Kit PDF manual download

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