PageRuler™ Prestained Protein Ladder, 10 to 180 kDa

描述Thermo Scientific PageRuler Prestained Protein Ladder is a mixture of 10 blue-, orange-, and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on SDS-polyacrylamide gels and western blots


For Research Use Only. Not for use in diagnostic procedures.图表
 

SDS-PAGE band profile of the PageRuler Prestained Protein Ladder

Migration patterns of the PageRuler Prestained Protein Ladder in different electrophoretic conditions

规格

Detection Method:	colorimetric, NIR fluorescence (700nm channel), RGB fluorescence (555nm channel)
Molecular Weight:	180, 130, 100, 70, 55, 40, 35, 25, 15, 10 kDa
Number of Markers:	10
Stain Type:	3 colors: Blue, Orange, Green
Size Range:	10 to 180 kDa
System:	SDS-PAGE
Product Line:	PageRuler™
Product Size:	2 x 250 µL

内容及储存Contents: two vials of 250 µL each

Storage buffer: 62.5 mM Tris-H₃PO₄ (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN₃ and 33% glycerol

Storage: Upon receipt store at -20°C