pTEF:ATP pTEF-ATP PVT11292 2ug
pTEF:ATP pTEF-ATP Information
Promoter: TEF1
Replicon: pUC
Prokaryotic resistance: ampicillin Amp
Screening marker: hereditary mycin G418
Clone strain: Escherichia coli DH5 alpha
Culture conditions: 37 C
pTEF:ATP pTEF-ATP Description
PTEF:ATP pTEF-ATP is a yeast ATP sensor plasmid. HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.
pTEF:ATP pTEF-ATP Sequence
LOCUS Exported 8554 bp ds-DNA circular SYN 26-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..8554
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 281..1187
/label=HO up
terminator 1205..1394
/gene="S. cerevisiae CYC1"
/label=CYC1 terminator
/note="transcription terminator for CYC1"
CDS complement(1427..3262)
/codon_start=1
/label=ATeam1.03
/translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
AHFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
EFVTAAIDMKTVKVNITTPDGPVYDADIEMVSVRAESGDLGILPGHIPTKAPLKIGAVR
LKKDGQTEMVAVSGGTVEVRPDHVTINAQAAETAEGIDKERAEAARQRAQERLNSQSDD
TDIRRAELALQRALNRLDVAGKANEFMDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQ
SKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELD
GDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHM
KQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNIL
GHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIE"
promoter complement(3280..3678)
/gene="S. cerevisiae TEF1"
/label=TEF1 promoter
/note="promoter for EF-1-alpha"
promoter 3761..4104
/label=TEF promoter
/note="Ashbya gossypii TEF promoter"
CDS 4105..4914
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
terminator 4920..5117
/label=TEF terminator
/note="Ashbya gossypii TEF terminator"
misc_feature 5178..5675
/label=HO down
promoter 6639..6743
/gene="bla"
/label=AmpR promoter
CDS 6744..7604
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 7775..8363
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
61 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
121 ggcaccccag gctttacact ttatgcttcc gcggctcgta tgttgtgtgg aattgtgagc
181 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccactag tccgaggcct
241 cgagatccga tatcgccgtg gcggccgcca gctgaagctt aattatcctg ggcacgagtg
301 aaacaaagct aaaaccttta tttagcatgg ccattgaatg taacaattat atatatcgca
361 agcacaaaaa atcaaggaga gagaactacc actttgttca tgtgtacaat gttcattatc
421 tccataagca aaaaaaaaaa aatagaaaac atatgctata aggttgatat tctcacgagt
481 aagcggcact tgctacttat tgacattgca gatttttggc tacagaaata gtatattaga
541 gattataatt gctaatcaaa tcaaaatata aaattagtaa accaaaccat ttataccctt
601 ccttagtagt tatggattgt tttttaatga tatttctgca aaccaaagaa agattgttat
661 ccagatagaa tttagttttg atattcattt ttttgttgaa gattgaacgc catatctggg
721 cctcataatt caaaagacgg tgccattatc ggtagcgttt cgcattgtac tggatttcag
781 aaatttcaca gttgatgaat cgaaaagaat ggtctcattg caacacgtaa ggttaagatg
841 tcccttttta ccattatagg caataaatga atcataaaac gaccgtatac tggtgaaata
901 gtagggagaa cgagtacctg tagtaaaaag tataaatcat agttaatcgg gcaatgtccc
961 tcgatcaagg agtattgtgt catgttcgag acaaacgcca acatttttgt ttcttttgga
1021 caaatgttgt ttgcatttat gatccgttat attttgatct aatgtagagt tgcacgtagt
1081 tcttactggc aaagaaatcg atgcatacca aaaaagaata aaggtgatat ttgatcttta
1141 ccgtttagtt ccaacgtaaa attgtgcctt tggacttaaa atggcgtcgt acgctgagct
1201 ggatcttcga gcgtcccaaa accttctcaa gcaaggtttt cagtataatg ttacatgcgt
1261 acacgcgtct gtacagaaaa aaaagaaaaa tttgaaatat aaataacgtt cttaatacta
1321 acataactat aaaaaaataa atagggacct agacttcagg ttgtctaact ccttcctttt
1381 cggttagagc ggatcttagc tagccgcggt accaagctta ctcgagtcat tcaatgttat
1441 gtctaatttt gaagttggcc ttgataccat ttttttgttt gtcagcagtg atgtagacgt
1501 tgtgagagtt ataattgtat tctaacttat gacctaaaat gttaccatct tctttaaaat
1561 caataccttt caattcaatt ctattgacca aagtgtcacc ttcaaatttt acttcagctc
1621 tggtcttgta attaccatcg tccttaaaga aaatagttct ttcttggaca taaccttcag
1681 gcattgcaga cttaaaaaag tcatgttgct tcatgtggtc aggatatcta gcgaagcatt
1741 gtaaaccgta acccaatgtt gtaactaatg taggccaagg aacaggtaac ttaccggtag
1801 tgcatatcaa tttcaaagtc aatttaccgt atgttgcgtc accttcacct tcaccactaa
1861 ctgaaaactt atgaccattg acgtcaccgt ctaattctac taaaataggg acgacacctg
1921 tgaataattc ttcaccttta ctaaccatac caccagaacc acccttgtat aattcatcca
1981 tacccaaagt aataccggca gcggtgacaa attctaataa aaccatatga tctctttttt
温馨提示：不可用于临床ZL。
pTEF:ATP pTEF- ATP/ pTEF:ATP pTEF- ATP质粒/ pTEF:ATP pTEF- ATP Plasmid
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100T omega质粒提取试剂盒/质粒小提/质粒中提/质粒大提
OMEGA质粒提取/质粒小提/质粒中提/质粒大提/无内毒素小提试剂盒...
微粒标准物质(粒度、颗粒) Micro particle size standard 微粒标准物质(粒度、颗粒)
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