pESC- HIS/ pESC- HIS质粒/ pESC- HIS Plasmid_详细资料

产品信息

货号： PVT11233 CAS号：无供应商： LSM Bio 数量：货源充足英文名： pESC- HIS 保质期： 6month 保存条件：负20摄氏度规格： 2ug

pESC-HIS
Catalog No. PVT11233
Packing 2ug

pESC-HIS Information

Function yeast expression plasmid

Resistance Amp

Promoter: GAL1, GAL10 promoter

Plasmid size: 6706bp

Screening marker: DH5 alpha

Clone strains: 37 DEG C, aerobic, LB

pESC-HIS Description

The pESC vectors are a series of epitope-tagging vectors for expression of eukaryotic genes in the yeast S. cerevisiae. Each vector contains GAL1 and GAL10 yeast promoters in opposing orientations. With these vectors you can introduce one or two cloned genes into a yeast host strain under the control of an inducible promoter. These vectors also feature an extensive polylinker sequence and the ability to generate end-specific RNA transcripts from T3 and T7 promoters. Each of the pESC vectors contains one of four different yeast-selectable markers (HIS3, TRP1, LEU2, or URA3) in the same vector backbone.

The pESC vectors contain DNA sequences coding for epitope peptides that can be specifically recognized by monoclonal antibodies. A sequence for the FLAG® epitope (DYKDDDDK) is located in the multiple cloning site (MCS) downstream of the GAL10 promoter; a sequence for the c-Myc epitope (EQKLISEEDL) is located in the MCS downstream of the GAL1 promoter. You can insert your gene of interest in front of the epitope sequence to generate C-terminal tagging or after the epitope sequence for N-terminal tagging. These tags allow the protein of interest to be studied without generating a specific antibody to that protein. The epitope-tagged fusion proteins can be studied in transformed cells using well-characterized antibodies.

• Express two different genes simultaneously in S. cerevisiae
• Proteins tagged with unique epitope tags
• Fast and easy immunoprecipitations

pESC-HIS Sequence

LOCUS Exported 6706 bp ds-DNA circular SYN 13-APR-2017

DEFINITION synthetic circular DNA

ACCESSION .

VERSION .

KEYWORDS pESC-HIS

SOURCE synthetic DNA construct

ORGANISM synthetic DNA construct

REFERENCE 1 (bases 1 to 6706)

AUTHORS .

TITLE Direct Submission

FEATURES Location/Qualifiers

source 1..6706

/organism="synthetic DNA construct"

/mol_type="other DNA"

promoter 317..503

/gene="S. cerevisiae HIS3"

/note="HIS3 promoter"

CDS 504..1163

/codon_start=1

/gene="S. cerevisiae HIS3"

/product="imidazoleglycerol-phosphate dehydratase, required

for histidine biosynthesis"

/note="HIS3"

/note="yeast auxotrophic marker"

/translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE

QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF

KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL

ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"

rep_origin complement(1423..1878)

/direction=LEFT

/note="f1 ori"

/note="f1 bacteriophage origin of replication; arrow

indicates direction of (+) strand synthesis"

terminator 1961..2126

/gene="S. cerevisiae ADH1"

/note="ADH1 terminator"

/note="transcription terminator for the S. cerevisiae

alcohol dehydrogenase 1 (ADH1) gene"

CDS complement(2274..2297)

/codon_start=1

/product="FLAG(R) epitope tag, followed by an enterokinase

cleavage site"

/note="FLAG"

/translation="DYKDDDDK"

promoter 2344..3008

/gene="GAL1 and GAL10 genes of S. cerevisiae"

/note="GAL1,10 promoter"

/note="divergent inducible promoter, regulated by Gal4"

protein_bind 2560..2677

/bound_moiety="Gal4"

/note="UAS"

/note="upstream activating sequence mediating

Gal4-dependent induction"

promoter 3019..3037

/note="T7 promoter"

/note="promoter for bacteriophage T7 RNA polymerase"

CDS 3055..3084

/codon_start=1

/product="Myc (human c-Myc oncogene) epitope tag"

/note="Myc"

/translation="EQKLISEEDL"

terminator 3114..3303

/gene="S. cerevisiae CYC1"

/note="CYC1 terminator"

/note="transcription terminator for CYC1"

rep_origin complement(3546..4134)

/direction=LEFT

/note="ori"

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

CDS complement(4305..5165)

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/note="AmpR"

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

promoter complement(5166..5270)

/gene="bla"

/note="AmpR promoter"

rep_origin 5297..6639

/note="2u ori"

/note="yeast 2u plasmid origin of replication"

protein_bind complement(6260..6307)

/bound_moiety="FLP recombinase from the Saccharomyces

cerevisiae 2u plasmid"

/note="FRT"

/note="FLP-mediated recombination occurs in the 8-bp core

温馨提示：不可用于临床ZL。

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