pUC-SPYNE
Catalog No. PVT11196
Packing 2ug
pUC-SPYNE Informaiton
Promoter: CaMV 35S promoter
Plasmid classification: plant series plasmid; plant report plasmid; dual fluorescent plasmid
Prokaryotic resistance: ampicillin Amp
Clonal strain: DH5 alpha
Culture conditions: 37 C, aerobic LB
Expression host: plant cells
5'sequencing primers: 35S:GACGCACAATCCCACTATCC
Primers for 3'sequencing: primers designed based on sequences
pUC-SPYNE Description
PUC-SPYNE plasmid is a double fluorescent plasmid.
pUC-SPYNE Multiple cloning site
pUC-SPYNE Sequence
LOCUS Exported 4356 bp ds-DNA circular SYN 08-NOV-2017
DEFINITION synthetic circular DNA
KEYWORDS pUC-SPYNE
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4356)
FEATURES Location/Qualifiers
source 1..4356
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(1..9)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 483..587
/gene="bla"
/note="AmpR promoter"
CDS 588..1448
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1619..2207
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 2495..2516
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2531..2561
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 2569..2585
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2593..2609
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter 3133..3478
/note="CaMV 35S promoter"
/note="strong constitutive promoter from cauliflower mosaic
virus"
misc_feature 3487..3570
/note="MCS"
CDS 3574..3603
/codon_start=1
/product="Myc (human c-Myc oncogene) epitope tag"
/note="Myc"
/translation="EQKLISEEDL"
CDS 3604..4071
/codon_start=1
/note="YFP-N"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
terminator 4088..4340
/note="NOS terminator"
/note="nopaline synthase terminator and poly(A) signal"
primer_bind complement(4349..4356)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
61 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
121 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
181 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
241 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
301 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
361 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
421 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
481 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
541 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
601 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
661 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
721 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
781 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
841 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
901 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
961 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
1021 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
1081 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
1141 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
1201 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
1261 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
1321 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
1381 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
1441 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
1501 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgac <
温馨提示：不可用于临床ZL。
pUC- SPYNE/ pUC- SPYNE质粒/ pUC- SPYNE Plasmid
P1788/pUC-SPYNE(R)173植物双分子荧光质粒
pUC-SPYNE植物BIFC双分子荧光质粒
pUC-SPYNE(R)173植物双分子荧光BIFC质粒
pUC-SPYNE(R)173植物双分子荧光质粒
pUC-SPYNE(R)173植物双分子荧光质粒
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