pMSCV- loxp- dsRed- loxp- eGFP- Puro- WPRE/ pMSCV- loxp- dsRed- loxp- eGFP- Puro- WPRE质粒/ pMSCV- loxp- dsRed- loxp- eGFP- Puro- WPRE Plasmid_详细资料

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货号： PVT11052 CAS号：无供应商： LSM Bio 数量：货源充足英文名： pMSCV- loxp- dsRed- loxp- eGFP- Puro- WPRE 保质期： 6month 保存条件：负20摄氏度规格： 2ug

pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE
Catalog No. PVT11052
Packing 2ug

pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE Information

Promoter: PGK

Replicon: pUC ori

Plasmid classification: virus series, reverse virus cloning cloning vector

Plasmid size: 8500bp

Prokaryotic resistance: Amp

Screening markers: Puro

Cloned strain: Stbl3

Culture conditions: 37 centigrade, aerobic LB

Expression host: mammalian cells

Induction mode: no induction, instantaneous expression

5'sequencing primers: primers designed according to sequence

3'sequencing primers: primers designed according to sequence

pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE Description

PMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE is Expression Retroviral, Mammalian, Cre/Lox carrier, its background carrier is MSCV, insert SEQ is inserted. loxp-dsRed-loxp-MCS-eGFP.

pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE Sequence

LOCUS Exported 8500 bp ds-DNA circular SYN 06-SEP-2016

DEFINITION synthetic circular DNA

ACCESSION .

VERSION .

KEYWORDS Untitled 13

SOURCE synthetic DNA construct

ORGANISM synthetic DNA construct

REFERENCE 1 (bases 1 to 8500)

AUTHORS .

TITLE Direct Submission

JOURNAL Exported Tuesday, September 6, 2016 from SnapGene Viewer 3.1.4

FEATURES Location/Qualifiers

source 1..8500

/organism="synthetic DNA construct"

/mol_type="other DNA"

misc_feature 580..921

/note="MESV Psi"

/note="packaging signal of murine embryonic stem cell

virus"

CDS 988..1404

/note="gag (truncated)"

/note="truncated Moloney murine leukemia virus (MMLV) gag

gene lacking the start codon"

protein_bind 1438..1471

/bound_moiety="Cre recombinase"

/note="loxP"

/note="Cre-mediated recombination occurs in the 8-bp core

sequence (GCATACAT)."

CDS 1506..2183

/codon_start=1

/product="noncytotoxic tetrameric variant of DsRed

fluorescent protein (Strack et al., 2008)"

/note="DsRed-Express2"

/note="mammalian codon-optimized"

/translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK

LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV

VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK

ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH

LFQ"

protein_bind 2208..2241

/bound_moiety="Cre recombinase"

/note="loxP"

/note="Cre-mediated recombination occurs in the 8-bp core

sequence (GCATACAT)."

CDS 2298..3017

/codon_start=1

/product="enhanced GFP"

/note="EGFP"

/note="mammalian codon-optimized"

/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK

VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL

EFVTAAGITLGMDELYK"

promoter 3027..3526

/note="PGK promoter"

/note="mouse phosphoglycerate kinase 1 promoter"

CDS 3547..4146

/codon_start=1

/gene="pac from Streptomyces alboniger"

/product="puromycin N-acetyltransferase"

/note="PuroR"

/note="confers resistance to puromycin"

/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER

VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA

AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS

APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"

misc_feature 4216..4804

/note="WPRE"

/note="woodchuck hepatitis virus posttranscriptional

regulatory element"

CDS complement(4687..4698)

/codon_start=1

/product="Factor Xa recognition and cleavage site"

/note="Factor Xa site"

/translation="IEGR"

LTR 4861..5376

/note="3' LTR"

/note="3' long terminal repeat from murine embryonic stem

cell virus"

primer_bind complement(5545..5561)

/note="M13 rev"

/note="common sequencing primer, one of multiple similar

variants"

protein_bind 5569..5585

/bound_moiety="lac repressor encoded by lacI"

/note="lac operator"

/note="The lac repressor binds to the lac operator to

inhibit transcription in E. coli. This inhibition can be

relieved by adding lactose or

isopropyl-beta-D-thiogalactopyranoside (IPTG)."

promoter complement(5593..5623)

/note="lac promoter"

/note="promoter for the E. coli lac operon"

protein_bind 5638..5659

/bound_moiety="E. coli catabolite activator protein"

/note="CAP binding site"

/note="CAP binding activates transcription in the presence

of cAMP."

rep_origin complement(5947..6535)

/direction=LEFT

温馨提示：不可用于临床ZL。

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