pMSCVpuro-IRES-EGFP/ pMSCVpuro-IRES-EGFP质粒/ pMSCVpuro-IRES-EGFP Plasmid

pMSCVpuro-IRES-EGFP

Catalog No. PVT11051
Packing 2ug
 

pMSCVpuro-IRES-EGFP Information

Replicon: pUC ori

Plasmid classification: virus series, reverse virus cloning cloning vector

Prokaryotic resistance: ampicillin Amp

Screening marker: puromycin Puro

Clone strain: Escherichia coli Stbl3

Culture conditions: 37 C, aerobic, LB

Expression host: lactation cells

Induction mode: no need to induce, transient expression, or stable expression.

Primers for 5'sequencing: primers designed based on sequences

Primers for 3'sequencing: primers designed based on sequences

 

pMSCVpuro-IRES-EGFP Description

Mouse stem cell virus (MSCV) vector is a retroviral vector derived from mouse embryonic stem cell virus (MesV) and LN. In transfected packaging cell lines, pmscv Puro is expressed instantaneously and stably, or integrates. The transcript contains extended viral packaging signal_+, purinomycin resistance genes and target genes. Vectors achieve stable, high-level gene expression in hematopoietic and embryonic stem cells through a specially designed 5 "long-terminal repeat (LTR). This sequence is a virus from the mouse stem cell gene, which differs from several point mutations in other retroviral vectors MoMuLV LTR and deletions. These changes enhance transcriptional activation and inhibit transcriptional inhibition of embryonic stem cells and embryonic cancer cells. As a result, LTR drives the constitutive expression of target genes at higher levels in stem cells or other mammalian cell lines. A polyclonal site where a gene can be cloned, the LTR. Puromycin resistant gene puror downstream of the mouse phosphoglycerol kinase (PKG) promoter, selects antibiotics in eukaryotic cells. Pmscvpuro also contains the origin of replication and Escherichia coli ampR genes in bacterial breeding and antibiotic selection.

 

pMSCVpuro-IRES-EGFP Sequence