pStat3-TA-luc Catalog No. PVT10815
Packing 2ug
pStat3-TA-luc Information
Function Mammal reporter plasmid
Promoter: SV40
Replicator: pUC ori, SV40 ori
Terminator: SV40 poly (A) signal
Plasmid classification: mammalian cells, signal pathway reporting vectors
Plasmid size: 5672bp
Prokaryotic resistance: Amp
Cloned strain: DH5 alpha
Culture conditions: 37 centigrade, aerobic LB
Expression host: mammalian cells
Induction mode: no induction, instantaneous expression
5'sequencing primers: primers designed according to sequence
3'sequencing primers: primers designed according to sequence
pStat3-TA-luc Description
PSTAT3-TA-luc can be transfected into cells by conventional cell transfection method. The biyuntian firefly firefly firefly firefly luciferase reporter gene assay kit detection (RG005/RG006) or dual luciferase reporter gene assay kit (RG027/RG028).
STAT3 reagent can be activated, can be used as a positive control pSTAT3-TA-luc report gene detection.
pStat3-TA-luc Sequence
LOCUS Exported 5672 bp ds-DNA circular SYN 04-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5672)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sunday, September 4, 2016 from SnapGene Viewer 3.1.4
FEATURES Location/Qualifiers
source 1..5672
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 109..140
/note="minP"
/note="minimal TATA-box promoter with low basal activity"
CDS 173..1825
/codon_start=1
/product="firefly luciferase"
/note="luciferase"
/note="synthetic luc2 version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
polyA_signal 1869..1990
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter 2184..2541
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2392..2527
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2572..3366
/codon_start=1
/product="aminoglycoside phosphotransferase"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3391..3439
/note="synthetic polyadenylation signal"
rep_origin complement(3766..4354)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4554..5414)
/codon_start=1
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 5519..5567
/note="synthetic polyadenylation signal"
misc_feature 5581..5672
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gccggtaccg ctagcctcga gtgcttcccg aacgttgctt cccgaacgtt
61 gcttcccgaa cgttgcttcc gaacgtagat ctgcagaagc ttagacacta gagggtatat
121 aatggaagct cgacttccag cttggcaatc cggtactgtt ggtaaagcca ccatggaaga
181 tgccaaaaac attaagaagg gcccagcgcc attctaccca ctcgaagacg ggaccgccgg
241 cgagcagctg cacaaagcca tgaagcgcta cgccctggtg cccggcacca tcgcctttac
301 cgacgcacat atcgaggtgg acattaccta cgccgagtac ttcgagatga gcgttcggct
361 ggcagaagct atgaagcgct atgggctgaa tacaaaccat cggatcgtgg tgtgcagcga
421 gaatagcttg cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg tggctgtggc
481 cccagctaac gacatctaca acgagcgcga gctgctgaac agcatgggca tcagccagcc
541 caccgtcgta ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc aaaagaagct
601 accgatcata caaaagatca tcatcatgga tagcaagacc gactaccagg gcttccaaag
661 catgtacacc ttcgtgactt cccatttgcc acccggcttc aacgagtacg acttcgtgcc
721 cgagagcttc gaccgggaca aaaccatcgc cctgatcatg aacagtagtg gcagtaccgg
781 attgcccaag ggcgtagccc taccgcaccg caccgcttgt gtccgattca gtcatgcccg
841 cgaccccatc ttcggcaacc agatcatccc cgacaccgct atcctcagcg tggtgccatt
901 tcaccacggc ttcggcatgt tcaccacgct gggctacttg atctgcggct ttcgggtcgt
961 gctcatgtac cgcttcgagg aggagctatt cttgcgcagc ttgcaagact ataagattca
1021 atctgccctg ctggtgccca cactatttag cttcttcgct aagagcactc tcatcgacaa
1081 gtacgaccta agcaacttgc acgagatcgc cagcggcggg gcgccgctca gcaaggaggt
1141 aggtgaggcc gtggccaaac gcttccacct accaggcatc cgccagggct acggcctgac
1201 agaaacaacc agcgccattc tgatcacccc cgaaggggac gacaagcctg gcgcagtagg
1261 caaggtggtg cccttcttcg aggctaaggt ggtggacttg gacaccggta agacactggg
1321 tgtgaaccag cgcggcgagc tgtgcgtccg tggccccatg atcatgagcg gctacgttaa
1381 caaccccgag gctacaaacg ctctcatcga caaggacggc tggctgcaca gcggcgacat
温馨提示：不可用于临床ZL。
pStat3- TA- luc/ pStat3- TA- luc质粒/ pStat3- TA- luc Plasmid
pTA- Luc/ pTA- Luc质粒/ pTA- Luc Plasmid
百欧博伟 质粒pIRF3-Luc
