pRSETB-mCherry Catalog No. PVT10624
Packing 2ug
pRSETB-mCherry Information
Function Fluorescence E.coli Plasmid
Resistance Amp
Screen mCherry
Strain DH5alpha
Culture temperature 37degrees centigrade
pRSETB-mCherry Sequence
LOCUS Exported 3562 bp ds-DNA circular SYN 12-JAN-2018
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..3562
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 20..38
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 70..92
/note="RBS"
CDS 112..129
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
CDS 133..165
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/note="T7 tag (gene 10 leader)"
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
CDS 169..192
/codon_start=1
/product="Xpress(TM) epitope tag, including an enterokinase
recognition and cleavage site"
/note="Xpress(TM) tag"
/translation="DLYDDDDK"
misc_feature 199..909
/note="mCherry"
terminator 977..1024
/note="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 1121..1576
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1602..1706
/gene="bla"
/note="AmpR promoter"
CDS 1707..2567
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2738..3326
/direction=RIGHT
/note="ori"
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
61 agaaataatt ttgtttaact ttaagaagga gatatacata tgcggggttc tcatcatcat
121 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac
181 gatgacgata aggatccgat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
241 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
301 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
361 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
421 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
481 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
541 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
601 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
661 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
721 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
781 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
841 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
901 tacaagtaag cttgatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
961 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
1021 tttgctgaaa ggaggaacta tatccggatc tggcgtaata gcgaagaggc ccgcaccgat
1081 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
1141 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
1201 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
1261 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
1321 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
1381 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
1441 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
1501 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
1561 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
1621 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
1681 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
1741 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
1801 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
1861 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
1921 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
1981 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
2041 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
2101 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
2161 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
2221 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
2281 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
2341 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
2401 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
2461 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
2521 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
2581 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
2641 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
2701 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
2761 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
温馨提示：不可用于临床ZL。
pRSETB- mCherry/ pRSETB- mCherry质粒/ pRSETB- mCherry Plasmid
pRSETB/ pRSETB质粒
pLVX- mCherry- C1/ pLVX- mCherry- C1质粒/ pLVX- mCherry- C1 Plasmid
pTriEx-mCherry-LOV2/ pTriEx-mCherry-LOV2质粒
pHDE-35s-Cas9-mCherry-UBQ/ pHDE-35s-Cas9-mCherry-UBQ质粒
NL4-3 mCherry Luciferase/ NL4-3 mCherry Luciferase质粒
pTRE3G-mCherry(pTRE3GmCherry)质粒载体
沪公网安备 31011502008050号
