pCDNA-4mtD3cpv Catalog No. PVT10530
Packing 2ug
pCDNA-4mtD3cpv Information
Function Other source plasmids
Promoter: CMV promoter
Replicator: ori, F1 ori, SV40 ori
Terminator: bGH poly (A) signal
Plasmid classification: gene library plasmid; other gene plasmids; other source plasmids.
Plasmid size: 7754bp
Prokaryotic resistance: ampicillin Amp (100 u g/ml)
Screening markers: neomycin Neo/G418
Cloned strains of Escherichia coli, DH10B and other Escherichia coli
Culture conditions: 37 centigrade, aerobic, LB
Expression host: 293T mammalian cells
Induction mode: no induction
pCDNA-4mtD3cpv Reference
Ca2+ indicators based on computationally redesigned calmodulin-peptide pairs.
pCDNA-4mtD3cpv Sequence
LOCUS Exported 7754 bp ds-DNA circular SYN 15-AUG-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..7754
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(83..943)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(944..1048)
/gene="bla"
/label=AmpR promoter
enhancer 1314..1693
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1694..1897
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 1942..1960
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 1974..2060
/label=COX8a
/note="human COX subunit 8a"
misc_feature 2069..4325
/label=4mtD3cpv
/note="4mtD3cpv second generation cameleon (calcium sensor)
targeted to mitochondria"
CDS 3804..4322
/codon_start=1
/product="N-terminal fragment of mVenus for use in
bimolecular fluorescence complementation (BiFC) (Kodama and
Hu, 2010)"
/label=VN173
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
ANFKIRHNIE"
promoter complement(4386..4404)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
polyA_signal 4430..4654
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 4700..5128
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5142..5471
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 5322..5457
/label=SV40 ori
/note="SV40 origin of replication"
CDS 5538..6332
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 6506..6627
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(7078..7666)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
61 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
121 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
181 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
241 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
301 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
361 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
421 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
481 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
541 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
601 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
661 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
721 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
781 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
841 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
901 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
961 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
温馨提示：不可用于临床ZL。
pcDNA-4mtD3cpv/ pcDNA-4mtD3cpv质粒/ pcDNA-4mtD3cpv Plasmid
pcDNAⅡ质粒载体
pcDNAⅠ质粒载体
pcDNA-Ikka-HA WT/ pcDNA-Ikka-HA WT质粒
pCDNA-dCas9/ pCDNA-dCas9质粒
pcDNA-K-GECO1/ pcDNA-K-GECO1质粒
pcDNA 3.1-c-His/myc哺乳动物真核表达质粒
P0763/pCDNA-dCas9-p300 Core哺乳编辑质粒
