HBV 1.3-mer WT replicon Catalog No. PVT10528
Packing 2ug
HBV 1.3-mer WT replicon Information
Function Other source plasmids
Replicon: ori
Plasmid classification: gene library plasmid; other gene plasmids; other source plasmids.
Plasmid size: 6821bp
Prokaryotic resistance: ampicillin Amp (100 u g/ml)
Cloned strains of Escherichia coli, DH5 A and other Escherichia coli
Culture conditions: 37 centigrade, aerobic, LB
HBV 1.3-mer WT replicon Reference
DDX3 DEAD-Box RNA helicase inhibits hepatitis B virus reverse transcription by incorporation into nucleocapsids.
HBV 1.3-mer WT replicon Sequence
LOCUS Exported 6821 bp ds-DNA circular SYN 15-AUG-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..6821
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 843..1688
/codon_start=1
/product="middle (pre-S2/S) envelope protein of hepatitis B
virus"
/label=HBsAg (pre-S2/S)
/note="hepatitis B surface antigen"
/translation="MQWNSTTFHQTLQDPRVRGLYFPAGGSSSGTVNPVLTTASPLSSI
FSRIGDPALNMENITSGFLGPLLVLQAGFFLLTRILTIPQSLDSWWTSLNFLGGTTVCL
GQNSQSPTSNHSPTSCPPTCPGYRWMCLRRFIIFLFILLLCLIFLLVLLDYQGMLPVCP
LIPGSSTTSTGPCRTCMTTAQGTSMYPSCCCTKPSDGNCTCIPIPSSWAFGKFLWEWAS
ARFSWLSLLVPFVQWFVGLSPTVWLSVIWMMWYWGPSLYSILSPFLPLLPIFFCLWVYI
"
promoter complement(2866..2884)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2903..2919)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 2927..2943
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2951..2981)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2996..3017
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(3305..3893)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4064..4924)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4925..5029)
/gene="bla"
/label=AmpR promoter
primer_bind 5503..5519
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 5527..5545
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
1 ccggagacta ctgttgttag acgacgaggc aggtccccta gaagaagaac tccctcgcct
61 cgcagacgaa ggtctcaatc gccgcgtcgc agaagatctc aatctcggga atctcaatgt
121 tagtattcct tggactcata aggtggggaa ctttactggg ctttattctt ctactgtacc
181 tgtctttaat cctcattgga aaacaccatc ttttcctaat atacatttac accaagacat
241 tatcaaaaaa tgtgaacagt ttgtaggccc actcacagtt aatgagaaaa gaagattgca
301 attgattatg cctgccaggt tttatccaaa ggttaccaaa tatttaccat tggataaggg
361 tattaaacct tattatccag aacatctagt taatcattac ttccaaacta gacactattt
421 acacactcta tggaaggcgg gtatattata taagagagaa acaacacata gcgcctcatt
481 ttgtgggtca ccatattctt gggaacaaga tctacagcat ggggcagaat ctttccacca
541 gcaatcctct gggattcttt cccgaccacc agttggatcc agccttcaga gcaaacaccg
601 caaatccaga ttgggacttc aatcccaaca aggacacctg gccagacgcc aacaaggtag
661 gagctggagc attcgggctg ggtttcaccc caccgcacgg aggccttttg gggtggagcc
721 ctcaggctca gggcatacta caaactttgc cagcaaatcc gcctcctgcc tccaccaatc
781 gccagtcagg aaggcagcct accccgctgt ctccaccttt gagaaacact catcctcagg
841 ccatgcagtg gaattccaca accttccacc aaactctgca agatcccaga gtgagaggcc
901 tgtatttccc tgctggtggc tccagttcag gaacagtaaa ccctgttctg actactgcct
961 ctcccttatc gtcaatcttc tcgaggattg gggaccctgc gctgaacatg gagaacatca
1021 catcaggatt cctaggaccc cttctcgtgt tacaggcggg gtttttcttg ttgacaagaa
1081 tcctcacaat accgcagagt ctagactcgt ggtggacttc tctcaatttt ctagggggaa
1141 ctaccgtgtg tcttggccaa aattcgcagt ccccaacctc caatcactca ccaacctctt
1201 gtcctccaac ttgtcctggt tatcgctgga tgtgtctgcg gcgttttatc atcttcctct
1261 tcatcctgct gctatgcctc atcttcttgt tggttcttct ggactatcaa ggtatgttgc
1321 ccgtttgtcc tctaattcca ggatcctcaa caaccagcac gggaccatgc cggacctgca
1381 tgactactgc tcaaggaacc tctatgtatc cctcctgttg ctgtaccaaa ccttcggacg
1441 gaaattgcac ctgtattccc atcccatcat cctgggcttt cggaaaattc ctatgggagt
1501 gggcctcagc ccgtttctcc tggctcagtt tactagtgcc atttgttcag tggttcgtag
1561 ggctttcccc cactgtttgg ctttcagtta tatggatgat gtggtattgg gggccaagtc
1621 tgtacagcat cttgagtccc tttttaccgc tgttaccaat tttcttttgt ctttgggtat
1681 acatttaaac cctaacaaaa caaagagatg gggttactct ctaaatttta tgggttatgt
1741 cattggatgt tatgggtcct tgccacaaga acacatcata caaaaaatca aagaatgttt
1801 tagaaaactt cctattaaca ggcctattga ttggaaagta tgtcaacgaa ttgtgggtct
1861 tttgggtttt gctgcccctt ttacacaatg tggttatcct gcgttgatgc ctttgtatgc
1921 atgtattcaa tctaagcagg ctttcacttt ctcgccaact tacaaggcct ttctgtgtaa
1981 acaatacctg aacctttacc ccgttgcccg gcaacggcca ggtctgtgcc aagtgtttgc
2041 tgacgcaacc cccactggct ggggcttggt catgggccat cagcgcatgc gtggaacctt
2101 ttcggctcct ctgccgatcc atactgcgga actcctagcc gcttgttttg ctcgcagcag
温馨提示：不可用于临床ZL。
HBV 1.3- mer WT replicon/ HBV 1.3- mer WT replicon质粒/ HBV 1.3- mer WT replicon Plasmid
Addgene质粒-HBV 1.3-mer WT replicon表达HBV病毒基因
P1178/HBV 1.3-mer WT replicon病毒基因质粒
HBV 1.3-mer WT replicon病毒基因质粒
HBV 1.3-mer WT replicon病毒基因质粒
百欧博伟 质粒HBV 1.3-mer WT replicon
100T omega质粒提取试剂盒/质粒小提/质粒中提/质粒大提
OMEGA质粒提取/质粒小提/质粒中提/质粒大提/无内毒素小提试剂盒...
微粒标准物质(粒度、颗粒) Micro particle size standard 微粒标准物质(粒度、颗粒)
微粒标准物质(粒度、颗粒) / 微粒标准物质(粒度、颗粒)
微粒标准物质(粒度、颗粒) / 微粒标准物质(粒度、颗粒) 数量均值粒径:1.98μm
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