1136- Desmoplakin-GFP
Catalog No. PVT10351
Packing 2ug
Function ORF expression plasmid
Resistance Kan
Screen Neo/G418
Strain DH5alpha
Culture temperature 37degrees centigrade,Aerobic, LB
Promoter: CMV promoter
Terminator: SV40 poly (A) signal
Plasmid Size: 13661bp
plasmid Label: C-EGFP
1136- Desmoplakin-GFP Description
1136-DESMOPLAKIN-GFP plasmid is a mammalian cell over expression plasmid, CMV promoter Drive Desmoplakin human gene and EGFP green fluorescent protein fusion expression.
1136- Desmoplakin-GFP Reference
Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin.
1136- Desmoplakin-GFP Sequence
LOCUS Exported 13661 bp ds-DNA circular SYN 25-OCT-2017
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13661)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..13661
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 68..371
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 372..575
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 704..982
/label=DP insert 5'region
misc_feature 983..9600
/label=Desmoplakin
CDS 9614..10333
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
polyA_signal 10456..10577
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(10584..11039)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 11066..11170
/gene="bla"
/label=AmpR promoter
promoter 11172..11529
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 11380..11515
/label=SV40 ori
/note="SV40 origin of replication"
CDS 11564..12358
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 12590..12637
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 12966..13554
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
601 agcgctaccg gactcagatc tcgagctcaa gcttcgaatt cgcggccgcg tcaacagccc
661 ctgctccttg ggcccctcca tgccatgccg taatctctcc cacccgacca acaccaacac
721 ccagctccga cgcagctcct ctgcgccctt gccgccctcc gagccacagc tttcctcccg
781 ctcctgcccc cggcccgtcg ccgtctccgc gctcgcagcg gcctcgggag ggcccaggta
841 gcgagcagcg acctcgcgag ccttccgcac tcccgcccgg ttccccggcc gtccgcctat
901 ccttggcccc ctccgctttc tccgcgccgg cccgcctcgc ttatgcctcg gcgctgagcc
961 gctctcccga ttgcccgccg acatgagctg caacggaggc tcccacccgc ggatcaacac
1021 tctgggccgc atgatccgcg ccgagtctgg cccggacctg cgctacgagg tgaccagcgg
1081 cggcgggggc accagcagga tgtactattc tcggcgcggc gtgatcaccg accagaactc
1141 ggacggctac tgtcaaaccg gcacgatgtc caggcaccag aaccagaaca ccatccagga
1201 gctgctgcag aactgctccg actgcttgat gcgagcagag ctcatcgtgc agcctgaatt
1261 gaagtatgga gatggaatac aactgactcg gagtcgagaa ttggatgagt gttttgccca
1321 ggccaatgac caaatggaaa tcctcgacag cttgatcaga gagatgcggc agatgggcca
1381 gccctgtgat gcttaccaga aaaggcttct tcagctccaa gagcaaatgc gagcccttta
1441 taaagccatc agtgtccctc gagtccgcag ggccagctcc aagggtggtg gaggctacac
1501 ttgtcagagt ggctctggct gggatgagtt caccaaacat gtcaccagtg aatgtttggg
1561 gtggatgagg cagcaaaggg cggagatgga catggtggcc tggggtgtgg acctggcctc
1621 agtggagcag cacattaaca gccaccgggg catccacaac tccatcggcg actatcgctg
1681 gcagctggac aaaatcaaag ccgacctgcg cgagaaatct gcgatctacc agttggagga
1741 ggagtatgaa aacctgctga aagcgtcctt tgagaggatg gatcacctgc gacagctgca
1801 gaacatcatt caggccacgt ccagggagat catgtggatc aatgactgcg aggaggagga
1861 gctgctgtac gactggagcg acaagaacac caacatcgct cagaaacagg aggccttctc
1921 catacgcatg agtcaactgg aagttaaaga aaaagagctc aataagctga aacaagaaag
1981 tgaccaactt gtcctcaatc agcatccagc ttcagacaaa attgaggcct atatggacac
温馨提示：不可用于临床ZL。
1136- Desmoplakin- GFP/ 1136- Desmoplakin- GFP质粒/ 1136- Desmoplakin- GFP Plasmid
P1602/1136- Desmoplakin-GFP人源基因质粒
1136- Desmoplakin-GFP人源基因质粒
1136- Desmoplakin-GFP人源基因质粒
1136- Desmoplakin-GFP人源基因哺乳表达质粒
Addgene质粒-1136- Desmoplakin-GFP人ORF哺乳表达
P2435/pSV40-Icam5-m（1136-2754bp）小鼠基因质粒
pSV40-Icam5-m（1136-2754bp）小鼠基因质粒
pSV40-Icam5-m（1136-2754bp）小鼠基因质粒
100T omega质粒提取试剂盒/质粒小提/质粒中提/质粒大提
OMEGA质粒提取/质粒小提/质粒中提/质粒大提/无内毒素小提试剂盒...
微粒标准物质(粒度、颗粒) Micro particle size standard 微粒标准物质(粒度、颗粒)
