pGEX-6p-1-tankyrase Search name
pGEX-6p-1-tankyrase,Plasmid pGEX-6p-1-tankyrase,pGEX-6p-1-tankyrase vector
pGEX-6p-1-tankyrase Information
Alias: NM_003747.2; ARTD5; PARP-5a; PARP
Prokaryotic resistance: ampicillin Amp
Cloning strain: Escherichia coli DH5alpha
Culture conditions: 37 C
Species human(H. sapiens)
pGEX-6p-1-tankyrase Description
PGEX-6p-1-tankyrase is a large intestinal expression plasmid of human gene.
pGEX-6p-1-tankyrase Sequence
LOCUS Exported 7489 bp ds-DNA circular SYN 30-NOV-2017
FEATURES Location/Qualifiers
source 1..7489
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 183..211
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 219..235
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 258..911
/codon_start=1
/product="glutathione S-transferase from Schistosoma
japonicum"
/label=GST
/translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
CDS 918..941
/codon_start=1
/product="recognition and cleavage site for human
rhinovirus 3C and PreScission proteases"
/label=HRV 3C site
/translation="LEVLFQGP"
misc_feature 951..3470
/label=tankyrase
promoter 3792..3896
/gene="bla"
/label=AmpR promoter
CDS 3897..4757
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4928..5516
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 5760..5837
/gene="lacI (mutant)"
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
CDS 5838..6920
/codon_start=1
/gene="lacI"
/product="lac repressor"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
protein_bind 6933..6954
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6969..6999
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7007..7023
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 7031..7047
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS 7043..7489
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGI"
primer_bind complement(7063..7079)
/label=M13 fwd
温馨提示：不可用于临床ZL。
pGEX- 6p- 1- tankyrase/ pGEX- 6p- 1- tankyrase质粒/ pGEX- 6p- 1- tankyrase Plasmid
P0644/pGEX-6p-1-tankyrase人源基因质粒
pGEX-6p-1-tankyrase人源基因质粒
pGEX-6p-1-tankyrase人源基因质粒
百欧博伟 质粒pGEX-6p-1-tankyrase
Addgene质粒-pGEX-6p-1-tankyrase人ORF大肠表达
PGEX-4T-1质粒价格，PGEX-4T-1质粒说明书
PGEX-4T-1质粒/PGEX-4T-1质粒
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