pYM39-EYFP Search name
pYM39-EYFP,Plasmid pYM39-EYFP,pYM39-EYFP vector
pYM39-EYFP Multiple cloning site
pYM39-EYFP Information
Promoter: SP6, TEF, T7
Replicon: ori
Terminator: ADH1
Plasmid classification: yeast series, Pichia pastoris expression vector
Plasmid size: 4946bp
Plasmid tagging: EYFP
Prokaryotic resistance: Amp
Clonal strain: DH5 alpha
Culture conditions: 37 C, aerobic LB
Expression host: yeast cells
5'sequencing primers: T7:TAATACGACTCACTATAGGG
Primers for 3'sequencing: primers designed based on sequences
pYM39-EYFP Sequence
LOCUS Exported 4946 bp ds-DNA circular SYN 19-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 10
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4946)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Monday, September 19, 2016 from SnapGene Viewer 3.2.1
FEATURES Location/Qualifiers
source 1..4946
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 79..795
/codon_start=1
/product="enhanced YFP"
/note="EYFP"
/note="mammalian codon-optimized"
/translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
CDS 802..828
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/note="HA"
/translation="YPYDVPDYA"
terminator 861..1048
/gene="S. cerevisiae ADH1"
/note="ADH1 terminator"
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
gene 1123..2479
/note="kanMX"
/note="yeast selectable marker conferring kanamycin
resistance (Wach et al., 1994)"
promoter 1123..1466
/note="TEF promoter"
/note="Ashbya gossypii TEF promoter"
CDS 1467..2276
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin"
/translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
terminator 2282..2479
/note="TEF terminator"
/note="Ashbya gossypii TEF terminator"
promoter complement(2584..2602)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
rep_origin complement(2860..3448)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3619..4479)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4480..4584)
/gene="bla"
/note="AmpR promoter"
promoter 4930..2
/note="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatccgg agcaggtgct
61 ggtgctggtg ctggagcaat gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
121 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
181 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
241 gtgccctggc ccaccctcgt gaccaccttc ggctacggcg tgcagtgctt cgcccgctac
301 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
361 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
421 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
481 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
541 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
601 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
661 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
721 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
781 gagctgtaca agtaaggatc ctatccatat gacgttccag attacgctgc tcagtgctag
841 ggcgcgccac ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag
901 ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat
961 tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagtatgagg
1021 tcgctcttat tgaccacacc tctaccggca gatccgctag ggataacagg gtaatataga
1081 tctgtttagc ttgcctcgtc cccgccgggt cacccggcca gcgacatgga ggcccagaat
1141 accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg tcgcccgtac
温馨提示：不可用于临床ZL。
pYM39- EYFP/ pYM39- EYFP质粒/ pYM39- EYFP Plasmid
pET28a-EYFP/ pET28a-EYFP质粒
pAAV-hSyn-eNpHR 3.0-EYFP/ pAAV-hSyn-eNpHR 3.0-EYFP质粒
EYFP质粒载体
pYM39-EYFP质粒
pCDNA3.1 EYFP H148Q- I152L/ pCDNA3.1 EYFP H148Q- I152L质粒/ pCDNA3.1 EYFP H148Q- I152L Plasmid
P0507/pYM39-EYFP毕赤酵母质粒
P1579/pCDNA3.1 EYFP H148Q-I152L哺乳荧光质粒
P0027/pET28a-EYFP大肠荧光质粒
P1668/pCDNA3.1-Ano1-m-T2A-EYFP-H148Q-I152L小鼠基因质粒
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