pAAV-MCS Search name
pAAV-MCS,Plasmid pAAV-MCS,pAAV-MCS vector
pAAV-MCS Information
Promoter: CMV
Replicator: pUC ori, F1 ori
Terminator: hGH poly (A) signal
Plasmid classification: virus series, adenovirus associated cloning vector
Plasmid size: 4650bp
Prokaryotic resistance: Amp
Cloned strain: Stbl3
Culture conditions: 37 centigrade, aerobic LB
Expression host: mammalian cells
Induction mode: no induction, instantaneous expression
5'sequencing primers: CMV-F:CGCAAATGGGCGGTAGGCGTG
3'sequencing primers: hGH-PA-R: CCAGCTTGGTTCCCAATAGA
pAAV-MCS Description
pAAV-MCS vector contains high levels of genes in mammalian cells.
pAAV-MCS Sequence
LOCUS Exported 4650 bp ds-DNA circular SYN 27-AUG-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 8
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4650)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Saturday, August 27, 2016 from SnapGene Viewer 3.1.4
FEATURES Location/Qualifiers
source 1..4650
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 1..141
/note="AAV2 ITR"
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
enhancer 221..524
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 525..727
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
polyA_signal 1397..1873
/note="hGH poly(A) signal"
/note="human growth hormone polyadenylation signal"
repeat_region 1913..2053
/note="AAV2 ITR"
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 2128..2583
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2865..2969
/gene="bla"
/note="AmpR promoter"
CDS 2970..3830
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4001..4589
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa
181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
301 ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag tatttacggt
361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
601 ttgacgtcaa tgggagtttg ttttgcacca aaatcaacgg gactttccaa aatgtcgtaa
661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
721 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
781 ccatagaaga caccgggacc gatccagcct ccgcggattc gaatcccggc cgggaacggt
841 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata
901 ggcccacaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac
961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc
1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata
1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta
1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt
1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg
1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattggg attcgaacat
1321 cgattgaatt ccccggggat cctctagagt cgacctgcag aagcttgcct cgagcagcgc
1381 tgctcgagag atctacgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct
1441 ggaagttgcc actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt
1501 gtctgactag gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg
1561 gcaagttggg aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc
1621 agtggcacaa tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc
1681 tcagcctccc gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt
1741 tttttggtag agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca
1801 ggtgatctac ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc
1861 cttccctgtc cttctgattt tgtaggtaac cacgtgcgga ccgagcggcc gcaggaaccc
1921 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
1981 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
2041 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
2101 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
2161 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
2221 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
2281 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
2341 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
2401 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
2461 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
2521 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
2581 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
2641 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
2701 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
2761 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
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温馨提示：不可用于临床ZL。
pAAV- MCS/ pAAV- MCS质粒/ pAAV- MCS Plasmid
pLEX- MCS/ pLEX- MCS质粒/ pLEX- MCS Plasmid
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百欧博伟 质粒pLVX-MCS-10×His-PGK-Puro
质粒pCMV-MCS
pAAV-MCS-Ppargc1a-m-FLAG-HA/ pAAV-MCS-Ppargc1a-m-FLAG-HA质粒
P0244/pAAV-MCS腺相关质粒
pAAV-MCS质粒载体
pAAV-MCS-Ppargc1a-m-FLAG-HA小鼠基因质粒
pAAV-MCS腺相关质粒
pAAV-MCS腺相关质粒
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