pGL4.73[hRluc / SV40] Search name
pGL4.73[hRluc /SV40],Plasmid pGL4.73[hRluc /SV40],pGL4.73[hRluc /SV40] vector
pGL4.73[hRluc / SV40] Information
Alias: pGL4.73
Promoter: SV40
Replicon: pUC ori, SV40 ori
Terminator: SV40 poly (A) signal
Plasmid classification: mammalian cells, signal pathway reporting vectors
Plasmid size: 3921bp
Prokaryotic resistance: ampicillin Amp
Clone strain: Escherichia coli DH5 alpha
Culture conditions: 37 C, aerobic, LB
Expression host: lactation cells
Induction mode: no need to induce, transient expression.
5'sequencing primers: RVP3:CTAGCAAAATAGGCTGTCCC
Primers for 3'sequencing: primers designed based on sequences
pGL4.73[hRluc/SV40] Description
pGL4.73[hRluc/SV40] encodes the luciferase reporter gene hRluc (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression.The pGL4.73[hRluc/SV40] Vector contains the hRluc reporter gene and an SV40 early enhancer/promoter and can be used as an expression control or a co-reporter vector.
pGL4.73[hRluc/SV40] Cloning site
pGL4.73[hRluc/SV40] Sequence
LOCUS Exported 3921 bp ds-DNA circular SYN 26-JUL-2013
DEFINITION SV40 promoter-containing internal control vector for strong
constitutive expression of Renilla luciferase.
ACCESSION AY738229
VERSION .
KEYWORDS pGL4.73[hRluc SV40]
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3921)
AUTHORS Promega
TITLE Direct Submission
JOURNAL Exported Sunday, September 4, 2016 from SnapGene Viewer 3.1.4
FEATURES Location/Qualifiers
source 1..3921
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
promoter 106..463
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 314..449
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 499..1434
/codon_start=1
/product="Renilla luciferase"
/note="hRluc"
/note="human codon-optimized"
/translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
polyA_signal 1475..1596
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(2015..2603)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2803..3663)
/codon_start=1
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 3768..3816
/note="synthetic polyadenylation signal"
misc_feature 3830..3921
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcagatct gcgcagcacc
61 atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag gcggaaagaa
121 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
181 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
241 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
301 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
361 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
421 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gcaatccggt
481 actgttggta aagccaccat ggcttccaag gtgtacgacc ccgagcaacg caaacgcatg
541 atcactgggc ctcagtggtg ggctcgctgc aagcaaatga acgtgctgga ctccttcatc
601 aactactatg attccgagaa gcacgccgag aacgccgtga tttttctgca tggtaacgct
661 gcctccagct acctgtggag gcacgtcgtg cctcacatcg agcccgtggc tagatgcatc
721 atccctgatc tgatcggaat gggtaagtcc ggcaagagcg ggaatggctc atatcgcctc
781 ctggatcact acaagtacct caccgcttgg ttcgagctgc tgaaccttcc aaagaaaatc
841 atctttgtgg gccacgactg gggggcttgt ctggcctttc actactccta cgagcaccaa
901 gacaagatca aggccatcgt ccatgctgag agtgtcgtgg acgtgatcga gtcctgggac
961 gagtggcctg acatcgagga ggatatcgcc ctgatcaaga gcgaagaggg cgagaaaatg
1021 gtgcttgaga ataacttctt cgtcgagacc atgctcccaa gcaagatcat gcggaaactg
1081 gagcctgagg agttcgctgc ctacctggag ccattcaagg agaagggcga ggttagacgg
1141 cctaccctct cctggcctcg cgagatccct ctcgttaagg gaggcaagcc cgacgtcgtc
1201 cagattgtcc gcaactacaa cgcctacctt cgggccagcg acgatctgcc taagatgttc
1261 atcgagtccg accctgggtt cttttccaac gctattgtcg agggagctaa gaagttccct
1321 aacaccgagt tcgtgaaggt gaagggcctc cacttcagcc aggaggacgc tccagatgaa
1381 atgggtaagt acatcaagag cttcgtggag cgcgtgctga agaacgagca gtaattctag
1441 agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga gtttggacaa
1501 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
1561 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
1621 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
1681 tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg agagccttca acccagtcag
1741 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat
1801 catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc actgactcgc
1861 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
1921 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
1981 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
2041 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
2101 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
2161 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
2221 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
2281 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
2341 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
2401 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
2461 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
温馨提示:不可用于临床ZL。