Lenti dcas VP64- Blast/ Lenti dcas VP64- Blast质粒/ Lenti dcas VP64- Blast Plasmid

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Lenti dcas VP64-Blast Information

Plasmid type: CRISPR/Cas9 vector; gene knockout vector; mammalian vector

High copy / low copy: high copy

Cloning method: BsiWI, EcoRI

Promoter: EF1

Carrier size: 14085 BP

5'sequencing primers and sequences: GTTTGGATCTTGGTTCATTCTCAAGCCTCAG

3'sequencing primers and sequences: cacatagcgtaaaaggagcaacatag

Carrier label: -

Vector resistance: ampicillin (Ampicilin)

Screening markers: Blasticidin

Cloned strain: Stbl3

Host cells (lines): mammalian cells

Note: Mutation D10A and N863A in Cas9

Stability: stable expression

Composition / inducible type: composition

Virus / non virus: slow disease

Use:CRISPR-CAS27-sgRNA plasmid

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