lentiGuide- Puro/ lentiGuide- Puro质粒/ lentiGuide- Puro Plasmid

Search name

Bacterial Resistance:Ampicillin
Growth Strain:

Use:CRISPR-CAS25-sgRNA plasmid

lentiGuide-Puro plasmid information

Vector backbone Custom
Backbone size w/o insert (bp) 9482
Vector type Mammalian Expression, Lentiviral, CRISPR
Selectable markers Puromycin

Gene/Insert name S. pyogenes sgRNA cassette
Alt name S. pyogenes CRISPR customizable RNA element
Species Synthetic
Insert Size (bp) 100
Promoter hU6

Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) Stbl3
Growth instructions Use SapI digest to check for unwanted recombination of lentiviral plasmid. Only amplify in RecA- bacteria (eg. Stbl3).
Copy number High Copy

Cloning method Restriction Enzyme
5′ cloning site BsmBI (not destroyed)
3′ cloning site BsmBI (not destroyed)
5′ sequencing primer hU6-F
3′ sequencing primer hGata4-rev (5'-ATTGTGGATGAATACTGCC-3')

Gene/Insert name Puromycin resistance
Alt name puromycin N-acetyl-transferase
Alt name PAC
Insert Size (bp) 600
Promoter Ef1-a

Cloning method Restriction Enzyme
5′ cloning site BsiWI (not destroyed)
3′ cloning site MluI (not destroyed)
5′ sequencing primer EF-1a-F
3′ sequencing primer WPRE-R

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