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Promoter: Mini-TK promoter

Replicon: pUC ori

Terminator: SV40 poly (A) signal

Plasmid classification: lactation serial plasmid; lactating reporter plasmid; luciferase plasmid

Plasmid size: 5539bp

Plasmid tagging: Luc

Prokaryotic resistance: Amp (100 g/ml)

Cloning strains: E. coli DH5 and E.

Culture conditions: 37 C, LB, aerobic.

Expression host: lactation cells

Induction mode: no need to induce, transient expression.

5'sequencing primers: M13F (TGTAAAACGACGGCCAGT)

3'sequencing primers: M13R (CAGGAAACAGCTATGACC)

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