品名 | anti- BRCA2 antibody |
货号 | FNab00944 |
规格 | 100µg |
状态 | liquid |
纯化方法 | Immunogen affinity purified |
纯度 | ≥95% as determined by SDS-PAGE |
宿主 | Rabbit |
亚型 | IgG |
存储 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 24 months(Avoid repeated freeze / thaw cycles.) |
背景介绍 | Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA(ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A(RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex(transcription and export complex 2) subunits PCID2 and SHFM1/DSS1, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability. Silencing of BRCA2 promotes R-loop accumulation at actively transcribed genes in replicating and non-replicating cells, suggesting that BRCA2 mediates the control of R-loop associated genomic instability, independently of its known role in homologous recombination(PubMed:24896180). |
免疫原 | breast cancer 2, early onset |
别称 | FACD, FANCD1, breast cancer 2, early onset, BROVCA2 |
分子量 | 384kd |
Uniprot ID | P51587 |
适用种属 | Human, Mouse, Rat |
验证应用 | ELISA, WB, IHC |
建议稀释比例 | WB: 1:200-1:1000; IHC: 1:20-1:200 |
图片 | | Immunohistochemistry of paraffin-embedded rat brain using FNab00944(BRCA2 antibody) at dilution of 1:50 |
| HeLa cells were subjected to SDS PAGE followed by western blot with FNab00944(BRCA2 antibody) at dilution of 1:300 |
注意事项 | BRCA2 antibody|BRCA2抗体仅供科研使用 |
产品链接 | https://www.fn-test.cn/product/FNab00944/ |