品名 | anti- DGCR8 C-Terminal antibody |
货号 | FNab02351 |
规格 | 100µg |
状态 | liquid |
纯化方法 | Immunogen affinity purified |
纯度 | ≥95% as determined by SDS-PAGE |
宿主 | Rabbit |
亚型 | IgG |
存储 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 24 months(Avoid repeated freeze / thaw cycles.) |
背景介绍 | Component of the microprocessor complex that acts as a RNA-and heme-binding protein that is involved in the initial step of microRNA(miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts(pri-miRNAs) to release precursor miRNA(pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs(PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer(of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding(PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994). Specifically recognizes and binds N6-methyladenosine(m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing(PubMed:25799998). Involved in the silencing of embryonic stem cell self-renewal(By similarity). |
免疫原 | DiGeorge syndrome critical region gene 8 |
别称 | C22orf12, DGCR8, DGCRK6, Gy1 |
分子量 | 120 kDa |
Uniprot ID | Q8WYQ5 |
适用种属 | Human, Mouse, Rat |
验证应用 | ELISA, WB, IHC, IF, IP, FC |
建议稀释比例 | WB: 1:200-1:2000; IP: 1:200-1:2000; IHC: 1:50-1:200; IF: 1:20-1:200 |
图片 | | Immunohistochemistry of paraffin-embedded human breast cancer using FNab02351(DGCR8 antibody) at dilution of 1:100 |
| HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab02351(DGCR8 antibody) at dilution of 1:1000 |
注意事项 | DGCR8 C-Terminal antibody|DGCR8 C-Terminal抗体仅供科研使用 |
产品链接 | https://www.fn-test.cn/product/FNab02351/ |