品名 | anti- CASP3 antibody |
货号 | FNab09938 |
规格 | 100µg |
状态 | liquid |
纯化方法 | protein A+G purified |
纯度 | ≥95% as determined by SDS-PAGE |
宿主 | Mouse |
亚型 | IgG1 |
存储 | 0.01MPBS pH 7.4,-20℃ for 24 months(Avoid repeated freeze / thaw cycles.) |
背景介绍 | Caspase 3, also named as CPP32, SCA-1 and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase(PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins(SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6,-7 and-9. Caspase 3 is involved in the cleavage of huntingtin. Caspase 3 can also form heterocomplex with other proteins and performs MW of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3. |
免疫原 | caspase 3, apoptosis-related cysteine peptidase |
别称 | Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1 |
分子量 | 32-35 kDa, 19 kDa, 17 kDa |
Uniprot ID | P42574 |
适用种属 | Human, Mouse, Rat |
验证应用 | ELISA, IHC, WB,IF |
建议稀释比例 | WB: 1:500-1:2000; IHC: 1:50-1:300; IF: 1:50-1:200 |
图片 | | Immunohistochemistry of paraffin-embedded human breast cancer using FNab09938(CASP3 antibody) at dilution of 1:200 |
| Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with FNab09938 (CASP3 antibody) at dilution of 1:3000 |
注意事项 | CASP3 antibody|CASP3抗体仅供科研使用 |
产品链接 | https://www.fn-test.cn/product/FNab09938/ |