Chicken OPG / Osteoprotegerin ELISA Kit (Sandwich ELISA) - LS-F24271

Chicken OPG / Osteoprotegerin ELISA Kit (Sandwich ELISA) - LS-F24271Catalog
Size
Price
LS-F24271-1
1 plate
$725 USD
Available Discounts
5% off 5+ units
Request Bulk
Add to Wish List
Add to Cart


SpecificationsPublicationsReviewsImagesProduct DescriptionLS-F24271 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Chicken OPG / Osteoprotegerin in samples of Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of OPG / Osteoprotegerin as low as 75 picograms per milliliter.

---

SpecificationsType
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
OPG / Osteoprotegerin
Reactivity
 
Chicken
Specificity
 
This kit recognizes natural and recombinant Chicken OPG.
Manual
 

Intended Sample Types
 
Plasma, Serum
Format
 
96-Well Microplate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
125 - 8000 pg/ml
Sensitivity
 
75 pg/ml
Assay Time
 
3.5 h
Precision
 
Intra-Assay: CV<10% Inter-Assay: CV<10%
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• User Manual
• 96-well Microplate
• Detection Reagents
• Wash Buffer
• Substrate
• Stop Solution
• Adhesive Plate Sealers

Protocol
 

1. Prepare all reagents, samples and standards.
2. Add 100 µl standard or sample to each well and incubate for 90 minutes at 37°C.
3. Aspirate and add 100 µl prepared Detection Antibody and incubate of 1 hour at 37°C.
4. Aspirate and wash 3 times.
5. Add 100 µl HRP Conjugate and incubate for 30 minutes at 37°C.
6. Aspirate and wash 5 times.
7. Add 90 µl Substrate Solution and incubate for 15-20 minutes at 37°C.
8. Add 50 µl Stop Solution.
9. Read immediately at 450nm.

Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio Guarantee.
About OPG / Osteoprotegerin
 
Uniprot: O00300 NCBI: NM_002546 NP_002537.3

 

Popular OPG / Osteoprotegerin Elisa Kits
Human OPG / Osteoprotegerin ELISA Kit (Sandwich ELISA) - LS-F2529
Reactivity: Human
Range: 93.7-6000 pg/ml

Rat OPG / Osteoprotegerin ELISA Kit (Sandwich ELISA) - LS-F12843
Reactivity: Rat
Range: 15.6-1000 pg/ml

Mouse OPG / Osteoprotegerin ELISA Kit (Sandwich ELISA) - LS-F279
Reactivity: Mouse
Range: 1.646-400 pg/ml

Rat OPG / Osteoprotegerin ELISA Kit (Sandwich ELISA) - LS-F5510
Reactivity: Rat
Range: 0.156-10 ng/ml

Pig OPG / Osteoprotegerin ELISA Kit (Sandwich ELISA) - LS-F21700
Reactivity: Pig
Range: 0.156-10 ng/ml
More OPG / Osteoprotegerin Products

---

Publications (0)Submit a Publication

---

Customer Reviews (0)Submit a Review
Top of Page

---

Images

 

 

Sandwich ELISA Platform Overview