Human Nesfatin-1 ELISA Kit (Competitive EIA) - LS-F40059

Human Nesfatin-1 ELISA Kit (Competitive EIA) - LS-F40059Catalog
Size
Price
LS-F40059-1
1 plate
$715 USD
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SpecificationsPublicationsReviewsImagesProduct DescriptionLS-F40059 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human Nesfatin-1 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Competitive EIA assay principle and can be used to detect levels of Nesfatin-1 as low as 0.188 nanograms per millilter.

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SpecificationsType
 
Competitive EIA (enzyme immunoassay) kit
Target
 
Nesfatin-1
Reactivity
 
Human
Manual
 

Intended Sample Types
 
Plasma, Serum, Tissue Homogenates
Format
 
96-Well Strip Plate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
0.312 - 20 ng/ml
Sensitivity
 
0.188 ng/ml
Precision
 
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
 
Store at 4°C. Stable for 6 months.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• User Manual
• 96-well Microplate
• Detection Reagents
• Wash Buffer
• Substrate
• Stop Solution
• Adhesive Plate Sealers

Background
 
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with NEFA. During the reaction, NEFA in the sample or standard competes with a fixed amount of NEFA on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to NEFA. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NEFA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio Guarantee.Popular Nesfatin-1 Elisa Kits
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Range: 617.28-50000 pg/ml

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Human Nesfatin-1 ELISA Kit (Competitive EIA) - LS-F10672
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Range: 0.312-20 ng/ml

Rat Nesfatin-1 ELISA Kit (Competitive EIA) - LS-F9739
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Range: 493.83-40000 pg/ml

Human Nesfatin-1 ELISA Kit (Sandwich ELISA) - LS-F22998
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Range: 15.625-1000 pg/ml
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