Human EpCAM / TROP-1 / TACSTD1 Protein (His Tag)

分子别名（Synonym）:DIAR5,EGP-2,EGP314,EGP40,ESA,HNPCC8,KS1/4,KSA,M4S1,MIC18,MK-1,TACSTD1,TROP-1,TROP1种属（Species）：Human表达系统（Expressed Host）：HEK293 Cells制剂（Buffer）：Lyophilized from sterile PBS, pH 7.4分子量（Molecule Mass）：The recombinant human EpCAM consists of 253 amino acids after removal of the signal peptide and has a calculated molecular mass of 29 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh EpCAM is approximately 36 kDa due to glycosylation.内毒素（Endotoxin）：＜1.0 EU per μg by the LAL method.生物活性(Activity)：Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When cells are added to EpCAM-His coated plates (1.25μg/mL, 100μL/well), approximately >40% will adhere specifically after 30 minutes at 37℃.表达区间（Protein Construction）：A DNA sequence encoding the extracellular domain (Ser 2-Lys265) of human EpCAM (NP_002345.1) was fused with a polyhistidine tag at the C-terminus.存储条件（Storage）：For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.运输条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.