Human IL-23 (IL23A & IL12B Heterodimer) Protein

分子别名（Synonym）:IL-23,IL-23A,IL23P19,P19,SGRF种属（Species）：Human表达系统（Expressed Host）：HEK293 Cells制剂（Buffer）：Lyophilized from sterile PBS, pH 7.4分子量（Molecule Mass）：The recombinant IL23 heterodimer of human IL23A/IL12B comprises 498 (181 + 317) amino acids and has a calculated molecular mass of 56.3 (20.1 + 36.2) kDa. The apparent molecular mass of rhIL23 heterodimer is approximately 22 & 45 kDa respectively in SDS-PAGE under reducing conditions.内毒素（Endotoxin）：＜1.0 EU per μg by the LAL method.生物活性(Activity)：1. Measured by its ability to bind biotinylated recombinant human IL12RB1 in a functional ELISA.表达区间（Protein Construction）：A DNA sequence encoding the IL23A (Q9NPF7) (Ser 2-Pro 189) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the p40 subunit of human IL12, termed as IL12B (NP_002178.2) (Ser 2-Ser 328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the IL23 heterodimer was purified.存储条件（Storage）：For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.运输条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.