Human NCL / Nucleolin ELISA Kit (Sandwich ELISA) - LS-F6791

Human NCL / Nucleolin ELISA Kit (Sandwich ELISA) - LS-F6791Catalog
Size
Price
LS-F6791-1
1 plate
$990 USD
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10% off 5+ units
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SpecificationsPublicationsReviewsImagesProduct DescriptionLS-F6791 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human NCL / Nucleolin in samples of Cell Lysates and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of NCL / Nucleolin as low as 0.06 nanograms per millilter.

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SpecificationsType
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
NCL / Nucleolin
Reactivity
 
Human
Specificity
 
Nucleolin (NCL) No significant cross-reactivity or interference between Nucleolin (NCL) and analogs was observed.
Manual
 

Intended Sample Types
 
Cell Lysates, Tissue Homogenates
Format
 
96-Well Strip Plate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
0.156 - 10 ng/ml
Sensitivity
 
0.06 ng/ml
Assay Time
 
4.5 h
Precision
 
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• User Manual
• 96-well Microplate
• Detection Reagents
• Wash Buffer
• Substrate
• Stop Solution
• Adhesive Plate Sealers

Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio Guarantee.
About NCL / Nucleolin
 
Uniprot: P19338 NCBI: NM_005381 NP_005372.2

 

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Sandwich ELISA Platform Overview