Mouse MMP1 ELISA Kit (Sandwich ELISA) - LS-F32461

Mouse MMP1 ELISA Kit (Sandwich ELISA) - LS-F32461Catalog
Size
Price
LS-F32461-1
1 plate
$865 USD
Request Bulk
Add to Wish List
Add to Cart


SpecificationsPublicationsReviewsImagesProduct DescriptionLS-F32461 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse MMP1 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of MMP1 as low as 75 picograms per milliliter.

---

SpecificationsType
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
MMP1
Reactivity
 
Mouse
Manual
 

Intended Sample Types
 
Plasma, Serum, Tissue Homogenates
Format
 
96-Well Strip Plate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
125 - 8000 pg/ml
Sensitivity
 
75 pg/ml
Precision
 
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
 
Store at 4°C. Stable for 6 months.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• User Manual
• 96-well Microplate
• Detection Reagents
• Wash Buffer
• Substrate
• Stop Solution
• Adhesive Plate Sealers

Background
 
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- MMP-1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MMP-1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MMP-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofMMP-1 can be calculated.
Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio Guarantee.
About MMP1
 
Uniprot: P03956 NCBI: NM_002421 NP_002412.1

 

Popular MMP1 Elisa Kits
Rabbit MMP1 ELISA Kit (Sandwich ELISA) - LS-F4708
Reactivity: Rabbit
Range: 31.25-2000 pg/ml

Human MMP1 ELISA Kit (Sandwich ELISA) - LS-F4960
Reactivity: Human
Range: 0.156-10 ng/ml

Rabbit MMP1 ELISA Kit (Sandwich ELISA) - LS-F21706
Reactivity: Rabbit
Range: 0.16-10 ng/ml

Human MMP1 ELISA Kit (CLIA) - LS-F26145
Reactivity: Human
Range: 41.2-30000 pg/ml

Mouse MMP1 ELISA Kit (Sandwich ELISA) - LS-F5523
Reactivity: Mouse
Range: 62.5-4000 pg/ml
More MMP1 Products

---

Publications (0)Submit a Publication

---

Customer Reviews (0)Submit a Review
Top of Page

---

Images

 

 

Sandwich ELISA Platform Overview