Human MIA / CD-RAP ELISA Kit (Sandwich ELISA) - LS-F24609

Human MIA / CD-RAP ELISA Kit (Sandwich ELISA) - LS-F24609Catalog
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LS-F24609-1
1 plate
$690 USD
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SpecificationsPublicationsReviewsImagesProduct DescriptionLS-F24609 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human MIA / CD-RAP in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of MIA / CD-RAP as low as 10 picograms per milliliter.

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SpecificationsType
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
MIA / CD-RAP
Reactivity
 
Human
Specificity
 
For the detection and quantification of endogenous levels of natural and/or recombinant Human MIA1 proteins.
Manual
 

Intended Sample Types
 
Cell Culture Supernatants, Plasma, Serum
Format
 
96-Well Strip Plate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
15.6 - 1000 pg/ml
Sensitivity
 
10 pg/ml
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• User Manual
• 96-well Microplate
• Detection Reagents
• Wash Buffer
• Substrate
• Stop Solution
• Adhesive Plate Sealers

Background
 
Melanoma Inhibiting Activity (MIA), also known as cartilage-derived retinoic acid-sensitive protein (CD-RAP), is an approximately 11-15 kDa protein that is expressed as a noncovalent homodimer. MIA is structurally related to OTOR/Otoraplin and MIA-2 in a small family of secreted proteins with one SH3 domain (1-3). And the MIA gene was mapped to 19q13.32-q13.33 by fluorescence in situ hybridization. Beside, It is a marker for malignant melanoma. MIA functions as a chemoattractant for mesenchymal stem cells and enhances their BMP-2 and TGF-ß3 induced differentiation into chondrocytes . MIA-deficient mice exhibit delayed chondrocyte differentiation but enhanced chondrocyte proliferation and cartilage repair .
Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio Guarantee.
About MIA / CD-RAP
 
Uniprot: Q16674 NCBI: NM_006533 NP_006524.1

 

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