pUC57-Simple-gRNA backbone爪蟾编辑质粒

质粒图谱


质粒序列
LOCUS       Exported                2845 bp ds-DNA     circular SYN 28-SEP-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pUC57-Simple-gRNA backbone
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 28, 2017 from Plasmid
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        234..252
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_RNA        271..346
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        888..992
                     /gene="bla"
                     /label=AmpR promoter
     CDS             993..1853
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2024..2612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
       61 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      121 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      181 caagctcgct tgcacgcctc tgcactcgtc ggtcccggca tccgataccc aattaatacg
      241 actcactata ggtcgtcttc gagaagacct gttttagagc tagaaatagc aagttaaaat
      301 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt aaagacccaa
      361 tatctcgacg cattcgcgaa gtaccgatct ccaattcact ggccgtcgtt ttacaacgtc
      421 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
      481 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
      541 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
      601 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
      661 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
      721 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
      781 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
      841 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
      901 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      961 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     1021 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     1081 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     1141 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     1201 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     1261 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     1321 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     1381 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     1441 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     1501 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     1561 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     1621 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     1681 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     1741 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     1801 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     1861 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     1921 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     1981 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2041 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2101 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     2161 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     2221 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     2281 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     2341 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     2401 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     2461 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     2521 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     2581 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     2641 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     2701 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     2761 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     2821 ccgcgcgttg gccgattcat taatg
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