pEarleyGate 301植物表达质粒

基本信息

终止子:	OCS terminator
质粒标签:	C-HA
原核抗性:	卡那霉素Kan
筛选标记:	草铵膦Bar
克隆菌株:	大肠杆菌DB3.1
培养条件:	37℃

质粒简介
          pEarleyGate 301(C-HA)是一个植物细胞Gateway系统表达质粒。pEarleyGate 301 allows for rapid recombinational cloning of DNA from Gateway entry vectors to be epitope tagged with HA tag at the C-terminus of the encoded protein. This vector allows for expression of genes from a promoter of choice rather than from the enhanced 35S promoter (see pEarleyGate 101-105 or 201-204). 质粒图谱
质粒序列
LOCUS       Exported               10379 bp ds-DNA     circular SYN 18-DEC-2017
DEFINITION  Gateway(R)-compatible promoterless plant transformation vector with 
            a C-terminal HA tag.
KEYWORDS    pEarleyGate 301
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10379)
  AUTHORS   Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS.
  TITLE     Gateway-compatible vectors for plant functional genomics and 
            proteomics.
  JOURNAL   Plant J. 2006;45:616-29.
  PUBMED    16441352
REFERENCE   2  (bases 1 to 10379)
  AUTHORS   Pikaard Lab
  TITLE     Direct Submission
COMMENT     The sequence was corrected by inserting a G at position 6487.
FEATURES             Location/Qualifiers
     source          1..10379
                     /organism="synthetic DNA construct"
                     /lab_host="Plant Cells"
                     /mol_type="other DNA"
     misc_feature    3..27
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      113..365
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(375..926)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=Bar
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(932..1312)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     protein_bind    1364..1483
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1513..1543
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1597..2256
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=Chl
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2598..2903
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2944..3068)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3070..3096
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      3150..3857
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    4101..4125
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5425..6054
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6483..7556
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid 
                     pVS1"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7622..7816
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8160..8300
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8486..9074)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9161..9955)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=Kan
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 gctggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
       61 cggacgtttt taatgtactg aattaacgcc gaattaattc gagctcggat ctgataattt
      121 atttgaaaat tcataagaaa agcaaacgtt acatgaattg atgaaacaat acaaagacag
      181 ataaagccac gcacatttag gatattggcc gagattactg aatattgagt aagatcacgg
      241 aatttctgac aggagcatgt cttcaattca gcccaaatgg cagttgaaat actcaaaccg
      301 ccccatatgc aggagcggat cattcattgt ttgtttggtt gcctttgcca acatgggagt
      361 ccaagattct gcagtcaaat ctcggtgacg ggcaggaccg gacggggcgg taccggcagg
      421 ctgaagtcca gctgccagaa acccacgtca tgccagttcc cgtgcttgaa gccggccgcc
      481 cgcagcatgc cgcggggggc atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg
      541 ggcagcccga tgacagcgac cacgctcttg aagccctgtg cctccaggga cttcagcagg
      601 tgggtgtaga gcgtggagcc cagtcccgtc cgctggtggc ggggggagac gtacacggtc
      661 gactcggccg tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg
      721 gcgacctcgc cgtccacctc ggcgacgagc cagggatagc gctcccgcag acggacgagg
      781 tcgtccgtcc actcctgcgg ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg
      841 atgtagtggt tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc acggcggatg
      901 tcggccgggc gtcgttctgg gctcatcgat tcgatttggt gtatcgagat tggttatgaa
      961 attcagatgc tagtgtaatg tattggtaat ttgggaagat ataataggaa gcaaggctat
     1021 ttatccattt ctgaaaaggc gaaatggcgt caccgcgagc gtcacgcgca ttccgttctt
     1081 gctgtaaagc gttgtttggt acacttttga ctagcgaggc ttggcgtgtc agcgtatcta
     1141 ttcaaaagtc gttaatggct gcggatcaag aaaaagttgg aatagaaaca gaatacccgc
     1201 gaaattcagg cccggttgcc atgtcctaca cgccgaaata aacgaccaaa ttagtagaaa
     1261 aataaaaact gactcggata cttacgtcac gtcttgcgca ctgatttgaa aaatctcaga
     1321 attgaattct gcagtcgacg gtaccgcggg cccgggatca tcaacaagtt tgtacaaaaa
     1381 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
     1441 aaaacagact acataatact gtaaaacaca acatatccag tcatattggc ggccgcatta
     1501 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
     1561 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     1621 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     1681 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     1741 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     1801 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     1861 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     1921 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     1981 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     2041 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     2101 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     2161 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
     2221 caacagtact gcgatgagtg gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa
     2281 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
     2341 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag
     2401 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
     2461 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
     2521 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
     2581 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
     2641 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
     2701 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
     2761 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
     2821 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     2881 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
     2941 gaccatagtg actggatatg ttgtgtttta cagcattatg tagtctgttt tttatgcaaa
     3001 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
     3061 aaagtggtgt acccatacga tgttccagat tacgcttgac taggtgagtc tagagagtta
     3121 attaagaccc gggactagtc cctagagtcc tgctttaatg agatatgcga gacgcctatg
     3181 atcgcatgat atttgctttc aattctgttg tgcacgttgt aaaaaacctg agcatgtgta
     3241 gctcagatcc ttaccgccgg tttcggttca ttctaatgaa tatatcaccc gttactatcg
     3301 tatttttatg aataatattc tccgttcaat ttactgattg taccctacta cttatatgta
     3361 caatattaaa atgaaaacaa tatattgtgc tgaataggtt tatagcgaca tctatgatag
     3421 agcgccacaa taacaaacaa ttgcgtttta ttattacaaa tccaatttta aaaaaagcgg
     3481 cagaaccggt caaacctaaa agactgatta cataaatctt attcaaattt caaaagtgcc
     3541 ccaggggcta gtatctacga cacaccgagc ggcgaactaa taacgctcac tgaagggaac
     3601 tccggttccc cgccggcgcg catgggtgag attccttgaa gttgagtatt ggccgtccgc
     3661 tctaccgaaa gttacgggca ccattcaacc cggtccagca cggcggccgg gtaaccgact
     3721 tgctgccccg agaattatgc agcatttttt tggtgtatgt gggccccaaa tgaagtgcag
     3781 gtcaaacctt gacagtgacg acaaatcgtt gggcgggtcc agggcgaatt ttgcgacaac
     3841 atgtcgaggc tcagcaggac ctgcaggcat gcaagcttgg cactggccgt cgttttacaa
     3901 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     3961 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     4021 agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt ttcccgcctt
     4081 cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc
     4141 gtttattaga ataacggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
     4201 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc
     4261 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt
     4321 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
     4381 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
     4441 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
     4501 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
     4561 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc
     4621 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac
     4681 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt
     4741 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg
     4801 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc
     4861 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg
     4921 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc
     4981 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc
     5041 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga
     5101 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt
     5161 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc
     5221 gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc
     5281 ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg
     5341 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa
     5401 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg
     5461 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat
     5521 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa
     5581 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc
     5641 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg
     5701 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata
     5761 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
     5821 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
     5881 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
     5941 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
     6001 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
     6061 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
     6121 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
     6181 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
     6241 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
     6301 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
     6361 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
     6421 agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
     6481 gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc
     6541 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac
     6601 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc
     6661 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt
     6721 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg
     6781 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag
     6841 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc
     6901 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
     6961 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
     7021 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
     7081 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
     7141 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
     7201 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg
     7261 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
     7321 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
     7381 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
     7441 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
     7501 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
     7561 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc
     7621 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
     7681 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
     7741 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
     7801 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta
     7861 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
     7921 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc
     7981 cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat
     8041 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     8101 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     8161 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
     8221 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     8281 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8341 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8401 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8461 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8521 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8581 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8641 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8701 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8761 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8821 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8881 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     8941 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9001 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     9061 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     9121 aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat tcatccagta
     9181 aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct
     9241 cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat
     9301 accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt
     9361 tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct
     9421 tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc
     9481 agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc
     9541 ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga
     9601 tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg
     9661 agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa
     9721 agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc
     9781 gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt
     9841 cattttctcc caccagctta tataccttag caggagacat tccttccgta tcttttacgc
     9901 agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt
     9961 atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac
    10021 gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc
    10081 aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg
    10141 gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat
    10201 catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg
    10261 agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg
    10321 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctgg